miRNAs and Genes Involved in the Interplay between Ocular Hypertension and Primary Open-Angle Glaucoma. Oxidative Stress, Inflammation, and Apoptosis Networks
Por:
Raga-Cervera J, Bolarin JM, Millan JM, Garcia-Medina JJ, Pedrola L, Abellán-Abenza J, Valero-Vello M, Sanz-González SM, O'Connor JE, Galarreta-Mira D, Bendala-Tufanisco E, Mayordomo-Febrer A, Pinazo-Durán MD and Zanón-Moreno V
Publicada:
1 jun 2021
Ahead of Print:
21 may 2021
Categoría:
Medicine (miscellaneous)
Resumen:
Glaucoma has no cure and is a sight-threatening neurodegenerative disease affecting more than 100 million people worldwide, with primary open angle glaucoma (POAG) being the most globally prevalent glaucoma clinical type. Regulation of gene expression and gene networks, and its multifactorial pathways involved in glaucoma disease are landmarks for ophthalmic research. MicroRNAs (miRNAs/miRs) are small endogenous non-coding, single-stranded RNA molecules (18-22 nucleotides) that regulate gene expression. An analytical, observational, case-control study was performed in 42 patients of both sexes, aged 50 to 80 years, which were classified according to: (1) suffering from ocular hypertension (OHT) but no glaucomatous neurodegeneration (ND) such as the OHT group, or (2) have been diagnosed of POAG such as the POAG group. Participants were interviewed for obtaining sociodemographic and personal/familial records, clinically examined, and their tear samples were collected and frozen at 80 degrees C until processing for molecular-genetic assays. Tear RNA extraction, libraries construction, and next generation sequencing were performed. Here, we demonstrated, for the first time, the differential expression profiling of eight miRNAs when comparing tears from the OHT versus the POAG groups: the miR-26b-5p, miR-152-3p, miR-30e-5p, miR-125b-2-5p, miR-224-5p, miR-151a-3p, miR-1307-3p, and the miR-27a-3p. Gene information was set up from the DIANA-TarBase v7, DIANA-microT-CDS, and TargetScan v7.1 databases. To build a network of metabolic pathways, only genes appearing in at least four of the following databases: DisGeNet, GeneDistiller, MalaCards, OMIM PCAN, UniProt, and GO were considered. We propose miRNAs and their target genes/signaling pathways as candidates for a better understanding of the molecular-genetic bases of glaucoma and, in this way, to gain knowledge to achieve optimal diagnosis strategies for properly identifying HTO at higher risk of glaucoma ND. Further research is needed to validate these miRNAs to discern the potential role as biomarkers involved in oxidative stress, immune response, and apoptosis for the diagnosis and/or prognosis of OHT and the prevention of glaucoma ND.
Filiaciones:
:
Hospital of Manises, 46940 Valencia, Spain
Bolarin JM:
Technological Centre of Information and Communication Technologies (CENTIC), 30100 Murcia, Spain
Millan JM:
Sequencing Service at the University and Polytechnic Hospital La Fe, 46026 Valencia, Spain
:
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, 46017 Valencia, Spain
Department of Ophthalmology, General University Hospital "Morales Meseguer", 30007 Murcia, Spain
Department of Ophthalmology and Optometry, University of Murcia, 30120 Murcia, Spain
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Pedrola L:
Sequencing Service at the University and Polytechnic Hospital La Fe, 46026 Valencia, Spain
Abellán-Abenza J:
Technological Centre of Information and Communication Technologies (CENTIC), 30100 Murcia, Spain
:
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, 46017 Valencia, Spain
:
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, 46017 Valencia, Spain
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Cellular and Molecular Ophthalmobiology Group, Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain
O'Connor JE:
Laboratory of Cytomics, Joint Research Unit Principe Felipe Research Center and University of Valencia, 46010 Valencia, Spain
Galarreta-Mira D:
University Clinic Hospital of Valladolid, 47003 Valladolid, Spain
:
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU/FISABIO, 46020 Valencia, Spain
Physiology Department, Faculty of Health Sciences, CEU University, Alfara del Patriarca, 46115 Valencia, Spain
Mayordomo-Febrer A:
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU/FISABIO, 46020 Valencia, Spain
Animal Medicine and Surgery Department, Veterinary Medicine Faculty, CEU University, Alfara del Patriarca, 46115 Valencia, Spain
:
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, 46017 Valencia, Spain
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Cellular and Molecular Ophthalmobiology Group, Department of Surgery, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain
:
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, 46017 Valencia, Spain
Spanish Net of Ophthalmic Research OFTARED RD16/0008/0022, Institute of Health Carlos III., 28029 Madrid, Spain
Faculty of Health Sciences, Valencian International University, 46002 Valencia, Spain
Green Published, gold
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