Specificity of staphylococcal phage and SaPI DNA packaging as revealed by integrase and terminase mutations
Por:
Ubeda, C, Olivarez, N, Barry, P, Wang, H, Kong, X, Matthews, A, Tallent, S, Christie, G and Novick, R
Publicada:
1 abr 2009
Resumen:
SaPI1 and SaPIbov1 are chromosomal pathogenicity islands in Staphylococcus aureus that carry tst and other superantigen genes. They are induced to excise and replicate by certain phages, are efficiently encapsidated in SaPI-specific small particles composed of phage virion proteins and are transferred at very high frequencies. In this study, we have analysed three SaPI genes that are important for the phage-SaPI interaction, int (integrase) terS (phage terminase small subunit homologue) and pif (phage interference function). SaPI1 int is required for SaPI excision, replication and packaging in a donor strain, and is required for integration in a recipient. A SaPI1 int mutant, following phage induction, produces small SaPI-specific capsids which are filled with partial phage genomes. SaPIbov1 DNA is efficiently packaged into full-sized phage heads as well as into SaPI-specific small ones, whereas SaPI1 DNA is found almost exclusively in the small capsids. TerS, however, determines DNA packaging specificity but not the choice of large versus small capsids. This choice is influenced by SaPIbov1 gene 12, which prevents phage DNA packaging into small capsids, and which is also primarily responsible for interference by SaPIbov1 with phage reproduction.
Filiaciones:
:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Olivarez, N:
Virginia Commonwealth Univ, Sch Med, Dept Microbiol & Immunol, Richmond, VA 23298 USA
Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA
Barry, P:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Wang, H:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Kong, X:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Matthews, A:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Tallent, S:
Virginia Commonwealth Univ, Sch Med, Dept Microbiol & Immunol, Richmond, VA 23298 USA
Christie, G:
Virginia Commonwealth Univ, Sch Med, Dept Microbiol & Immunol, Richmond, VA 23298 USA
Novick, R:
NYU, Med Ctr, Kimmel Ctr Biol & Med, Skirball Inst,Program Mol Pathogenesis, New York, NY 10016 USA
NYU, Med Ctr, Dept Microbiol, New York, NY 10016 USA
NYU, Med Ctr, Dept Biochem, New York, NY 10016 USA
Green Accepted, Bronze
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