Integrative transnational analysis to dissect tuberculosis transmission events along the migratory route from Africa to Europe


Por: Martínez-Lirola M, Jajou R, Mathys V, Martin A, Cabibbe AM, Valera A, Sola-Campoy PJ, Abascal E, Rodríguez-Maus S, Garrido-Cárdenas JA, Bonillo M, Chiner-Oms Á, López B, Vallejo-Godoy S, Comas I, Prof PM, Prof DMC, Soolingen Prof D, Pérez-Lago L and de Viedma DG

Publicada: 1 may 2021 Ahead of Print: 1 abr 2021
Resumen:
Background: Growing international migration has increased the complexity of tuberculosis transmission patterns. Italy's decision to close its borders in 2018 made of Spain the new European porte entree for migration from the Horn of Africa (HA). In one of the first rescues of migrants from this region at the end of 2018, tuberculosis was diagnosed in eight subjects, mainly unaccompanied minors. Methods: Mycobacterium tuberculosis isolates from these recently arrived migrants were analysed by Mycobacterial Interspersed Repetitive-Unit/Variable-Number of Tandem Repeat (MIRU-VNTR) and subsequent whole genome sequencing (WGS) analysis. Data were compared with those from collections from other European countries receiving migrants from the HA and a strain-specific PCR was applied for a fast searching of common strains. Infections in a cellular model were performed to assess strain virulence. Results: MIRU-VNTR analysis allowed identifying an epidemiological cluster involving three of the eight cases from Somalia (0 single-nucleotide polymorphisms between isolates, HA cluster). Following detailed interviews revealed that two of these cases had shared the same migratory route in most of the trip and had spent a long time at a detention camp in Libya. To confirm potential en route transmission for the three cases, we searched the same strain in collections from other European countries receiving migrants from the HA. MIRU-VNTR, WGS and a strainspecific PCR for the HA strain were applied. The same strain was identified in 12 cases from Eritrea diagnosed soon after their arrival in 2018 to the Netherlands, Belgium and Italy. Intracellular replication rate of the strain did not reveal abnormal virulence. Conclusions: Our study suggests a potential en route transmission of a pan-susceptible strain, which caused at least 15 tuberculosis cases in Somalian and Eritrean migrants diagnosed in four different European countries.

Filiaciones:
Martínez-Lirola M:
 Complejo Hospitalario Torrecárdenas, Almería, Spain

Jajou R:
 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands

Mathys V:
 Unit Bacterial Diseases Service, Infectious Diseases in Humans, Sciensano, Brussels, Belgium

Martin A:
 Université catholique de Louvain (UCLouvain), Institute of Experimental and Clinical Research, 1200 Brussels, Belgium

 Current affiliation: Syngulon, 4102, Seraing, Belgium

Cabibbe AM:
 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

Valera A:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

Sola-Campoy PJ:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

Abascal E:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

Rodríguez-Maus S:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

Garrido-Cárdenas JA:
 Department of Biology and Geology, Universidad de Almería, Almería, Spain

Bonillo M:
 Unidad de Prevención, Promoción y Vigilancia de la Salud del Área Sanitaria Norte de Almería. Consejería de Salud. Junta de Andalucia, Spain

:
 Centro Superior de Investigación en Salud Pública (FISABIO)-Universitat de València, Valencia, Spain

López B:
 UPPV Distrito Sanitario Granada metropolitano, Granada, Consejería de Salud, Junta de Andalucía

Vallejo-Godoy S:
 Unidad de TB de Poniente, Almería

:
 Instituto de Biomedicina de Valencia-CSIC, Valencia, Spain

 CIBER Salud Pública (CIBERESP), Spain

Prof PM:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

 CIBER Enfermedades respiratorias (CIBERES), Spain

 Departamento de Medicina, Universidad Complutense, Madrid, Spain

Prof DMC:
 Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

Soolingen Prof D:
 National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands

Pérez-Lago L:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

de Viedma DG:
 Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

 Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

 CIBER Enfermedades respiratorias (CIBERES), Spain
ISSN: 11951982





JOURNAL OF TRAVEL MEDICINE
Editorial
Oxford University Press, England, Estados Unidos America
Tipo de documento: Article
Volumen: 28 Número: 4
Páginas:
WOS Id: 000732800900022
ID de PubMed: 33822988
imagen Green Submitted

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