Increased E2F1 mRNA and miR-17-5p Expression Is Correlated to Invasiveness and Proliferation of Pituitary Neuroendocrine Tumours
Por:
Garcia-Martinez, A, Lopez-Munoz, B, Fajardo, C, Camara, R, Lamas, C, Silva-Ortega, S, Aranda, I and Pico, A
Publicada:
1 abr 2020
Categoría:
Clinical biochemistry
Resumen:
miR-17-5p and E2F1 have been described as deregulated in cancer, but they have scarcely been studied in pituitary neuroendocrine tumours (PitNETs). This study evaluates the relationship of E2F1 and miR-17-5p with the invasiveness and proliferation of PitNETs. In this cross-sectional descriptive study, we evaluated the expression of E2F1, MYC, and miR-17-5p by quantitative real time PCR analysis in 60 PitNETs: 29 gonadotroph (GT), 15 functioning somatotroph (ST), and 16 corticotroph (CT) tumours, of which 8 were silent (sCT). The clinical data were collected from the Spanish Molecular Register of Pituitary Adenomas (REMAH) database. We defined invasiveness according to the Knosp classification and proliferation according to a molecular expression of Ki-67 >= 2.59. E2F1 was more expressed in invasive than in non-invasive tumours in the whole series (p = 0.004) and in STs (p = 0.01). In addition, it was overexpressed in the silent subtypes (GTs and sCTs; all macroadenomas) and normoexpressed in the functioning ones (fCTs and STs; some microadenomas). miR-17-5p was more expressed in proliferative than in non-proliferative tumours (p = 0.041) in the whole series but not by subtypes. Conclusions: Our study suggests that in PitNETs, E2F1 could be a good biomarker of invasiveness, and miR-17-5p of proliferation, helping the clinical management of these tumours.
Filiaciones:
Garcia-Martinez, A:
Hosp Gen Univ Alicante Inst Hlth & Biomed Res ISA, Res Lab, Alicante 03010, Spain
Lopez-Munoz, B:
Hosp Gen Univ Alicante ISABIAL, Dept Endocrinol & Nutr, Alicante 03010, Spain
:
Hosp La Ribera, Dept Endocrinol & Nutr, Valencia 46600, Spain
Camara, R:
Hosp Univ & Politecn La Fe, Dept Endocrinol & Nutr, Valencia 46026, Spain
Lamas, C:
Hosp Gen Univ Albacete, Dept Endocrinol & Nutr, Albacete 02006, Spain
Silva-Ortega, S:
Hosp Gen Univ Alicante ISABIAL, Dept Pathol, Alicante 03010, Spain
Aranda, I:
Hosp Gen Univ Alicante ISABIAL, Dept Pathol, Alicante 03010, Spain
Pico, A:
Miguel Hernandez Univ, Hosp Gen Univ Alicante, Dept Endocrinol & Nutr, Alicante 03010, Spain
Green Published, gold
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