Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
Por:
Jiménez-Ruano AC, Madrazo-Moya CF, Cancino-Muñoz I, Mejía-Ponce PM, Licona-Cassani C, Comas I, Muñiz-Salazar R and Zenteno-Cuevas R
Publicada:
21 ene 2021
Ahead of Print:
21 ene 2021
Categoría:
Multidisciplinary
Resumen:
Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to identify and characterize the lineages and genomic transmission clusters present in 67 DR-TB isolates circulating in southeastern Mexico. The results show the presence of three major lineages: L1 (3%), L2 (3%) and L4 (94%), the last one included 16 sublineages. Sublineage 4.1.1.3 (X3) was predominant in 18 (27%) of the isolates, including one genomic cluster, formed by eleven multidrug resistant isolates and sharing the SIT 3278, which seems to be restricted to Mexico. By the use of WGS, it was possible to identify the high prevalence of L4 and a high number of sublineages circulating in the region, also was recognized the presence of a novel X3 sublineage, formed exclusively by multidrug resistant isolates and with restrictive circulation in Mexico for at least the past 17 years.
Filiaciones:
Jiménez-Ruano AC:
Programa de Maestría en Ciencias de la Salud, Instituto de Ciencias de la Salud, Universidad Veracruzana, Xalapa, Veracruz, México
Instituto de Salud Pública, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Animas, 91190, Xalapa, Veracruz, México
Madrazo-Moya CF:
Programa de Maestría en Ciencias de la Salud, Instituto de Ciencias de la Salud, Universidad Veracruzana, Xalapa, Veracruz, México
Biomedical Institute of Valencia IBV-CSIC, Valencia, Spain
Cancino-Muñoz I:
Biomedical Institute of Valencia IBV-CSIC, Valencia, Spain
Mejía-Ponce PM:
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, Nuevo Leon, Mexico
Licona-Cassani C:
Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey, Nuevo Leon, Mexico
:
Biomedical Institute of Valencia IBV-CSIC, Valencia, Spain
CIBER of Epidemiology and Public Health, Madrid, Spain
Muñiz-Salazar R:
Laboratorio de Epidemiología y Ecología y Molecular, Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Ensenada, Baja California, México
Red Multidisciplinaria de Investigación en Tuberculosis, Mexico City, Mexico
Zenteno-Cuevas R:
Programa de Maestría en Ciencias de la Salud, Instituto de Ciencias de la Salud, Universidad Veracruzana, Xalapa, Veracruz, México.
Instituto de Salud Pública, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, A.P. 57, Col. Industrial Animas, 91190, Xalapa, Veracruz, México.
Red Multidisciplinaria de Investigación en Tuberculosis, Mexico City, Mexico.
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