A body weight loss- and health-promoting gut microbiota is established after bariatric surgery in individuals with severe obesity
Por:
Sanchez-Carrillo S, Ciordia S, Rojo D, Zubeldia-Varela E, Méndez-García C, Martínez-Martínez M, Barbas C, Ruiz-Ruiz S, Moya A, Garriga M, Salazar N, Botella-Carretero JI, Vega-Piñero B, de Los Reyes-Gavilán CG, Del Campo R and Ferrer M
Publicada:
30 ene 2021
Ahead of Print:
6 nov 2020
Resumen:
Obesity has reached an epidemic level worldwide, and bariatric surgery (BS) has been proven to be the most efficient therapy to reduce severe obesity-related comorbidities. Given that the gut microbiota plays a causal role in obesity development and that surgery may alter the gut environment, investigating the impact of BS on the microbiota in the context of severe obesity is important. Although, alterations at the level of total gut bacteria, total gene content and total metabolite content have started to be disentangled, a clear deficit exists regarding the analysis of the active fraction of the microbiota, which is the fraction that is most reactive to the BS. Here, active gut microbiota and associated metabolic functions were evaluated using shotgun proteomics and metabolomics in 40 severely obese volunteers. Samples from each volunteer were obtained under basal conditions, after a short high protein and calorie-restricted diet, and 1 and 3 months after BS, including laparoscopic surgery through Roux-en-Y Gastric Bypass or Sleeve Gastrectomy. The results revealed for the first time the most active microbes and metabolic flux distribution pre- and post-surgery and deciphered main differences in the way sugars and short-fatty acids are metabolized, demonstrating that less energy-generating and anaerobic metabolism and detoxification mechanisms are promoted post-surgery. A comparison with non-obese proteome data further signified different ways to metabolize sugars and produce short chain fatty acids and deficiencies in proteins involved in iron transport and metabolism in severely obese individuals compared to lean individuals. (C) 2020 Elsevier B.V. All rights reserved.
Filiaciones:
Sanchez-Carrillo S:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain
Ciordia S:
Unidad de Proteómica, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), 28049, Madrid, Spain
Rojo D:
Centre for Metabolomics and Bioanalysis (CEMBIO), Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid, Spain
Zubeldia-Varela E:
Centre for Metabolomics and Bioanalysis (CEMBIO), Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid, Spain
Departamento de Ciencias Médicas Básicas, Facultad de Medicina, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid, Spain
Méndez-García C:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain
Martínez-Martínez M:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain
Barbas C:
Centre for Metabolomics and Bioanalysis (CEMBIO), Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid, Spain
:
Unidad Mixta de Investigación en Genómica y Salud, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), 46020, València, Spain
CIBER en Epidemiología y Salud Pública (CIBERESP), 28029, Madrid, Spain
Instituto de Biología Integrativa de Sistemas, Universitat de València and Consejo Superior de Investigaciones Científicas (CSIC), 46980, València, Spain
:
Unidad Mixta de Investigación en Genómica y Salud, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), 46020, València, Spain
CIBER en Epidemiología y Salud Pública (CIBERESP), 28029, Madrid, Spain
Instituto de Biología Integrativa de Sistemas, Universitat de València and Consejo Superior de Investigaciones Científicas (CSIC), 46980, València, Spain
Garriga M:
Servicio de Endocrinología y Nutrición, Hospital Universitario Ramón y Cajal & IRyCIS, 28034, Madrid, Spain
Salazar N:
Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300, Villaviciosa, Asturias, Spain
Diet, Human Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), 33011, Oviedo, Spain
Botella-Carretero JI:
Servicio de Endocrinología y Nutrición, Hospital Universitario Ramón y Cajal & IRyCIS, 28034, Madrid, Spain
CIBER de Fisiopatología de la Obesidad y Nutrición (CIBERobn), 28029, Madrid, Spain
Vega-Piñero B:
Servicio de Endocrinología y Nutrición, Hospital Universitario Ramón y Cajal & IRyCIS, 28034, Madrid, Spain
de Los Reyes-Gavilán CG:
Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300, Villaviciosa, Asturias, Spain
Diet, Human Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), 33011, Oviedo, Spain
Del Campo R:
Servicio de Microbiología, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Universitario Ramón y Cajal, 28034, Madrid, Spain. Electronic address:
Ferrer M:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain
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