HLA-G as a new tumor biomarker: detection of soluble isoforms of HLA-G in the serum and saliva of patients with colorectal cancer
Por:
Lázaro-Sánchez AD, Salces-Ortiz P, Velásquez LI, Orozco D, Díaz-Fernández N and Juárez-Marroquí A
Publicada:
1 jul 2020
Ahead of Print:
20 nov 2019
Resumen:
Introduction Recent medical investigations suggest that HLA-G, due to its tolerogenic properties, can be used as a biomarker in the diagnosis, treatment, and prognosis of different neoplasms. This observational prospective pilot study aims at detecting sHLA-G in the serum and saliva of patients diagnosed with colorectal cancer (CRC). For this purpose, we compared the expression of sHLA-G from patients with a control sample from a healthy population. Materials and methods Using the specific enzyme-linked immunosorbent assay (ELISA) method, the expression of sHLA-G in the serum and saliva samples from patients affected by CRC (n = 20) and in a control sample (n = 10) were analyzed. Results The data showed that in patients with CRC, salivary sHLA-G values were significantly higher than in the control group (18.84 U/ml versus 6.3 U/ml,p = 0.036). In addition, higher levels of sHLA-G were observed in the saliva of patients with CRC in more advanced stages, compared with patients in early stages (24.2 U/ml vs. 8.1 U/ml,p = 0.019). A significant correlation was observed between the concentration of sHLA-G in the serum and saliva of the analyzed samples (Spearman correlation 0.7,p = 0.004). Conclusions This study demonstrates, for the first time, the possibility of detecting sHLA-G in the saliva of patients with CRC, resulting in a less invasive alternative to venipuncture. Likewise, we propose that sHLA-G could be an attractive molecular target based on its significant high levels in advanced stages.
Filiaciones:
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Medical Oncology Service of the University Hospital of Sant Joan d'Alacant, Ctra. Nnal. 332, s/n, 03550, Alicante, Spain.
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Medical Oncology Service of the University Hospital of Sant Joan d'Alacant, Ctra. Nnal. 332, s/n, 03550, Alicante, Spain
Velásquez LI:
Department of Clínical Medicine of the Miguel Hernández University of Elche, Alicante, Spain
Orozco D:
Department of Clínical Medicine of the Miguel Hernández University of Elche, Alicante, Spain
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Medical Oncology Service of the University Hospital of Sant Joan d'Alacant, Ctra. Nnal. 332, s/n, 03550, Alicante, Spain
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Medical Oncology Service of the University Hospital of Sant Joan d'Alacant, Ctra. Nnal. 332, s/n, 03550, Alicante, Spain
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