The Domestic Environment and the Lung Mycobiome
Por:
Rubio-Portillo E, Orts D, Llorca E, Fernández C, Antón J, Ferrer C, Gálvez B, Esteban V, Revelles E, Pérez-Martín C, Gómez-Imbernón E, Adsuar J, Piqueras P, Amat B, Franco J and Colom MF
Publicada:
1 nov 2020
Ahead of Print:
2 nov 2020
Resumen:
This study analyzes the relationship between the mycobiome of the Lower Respiratory Tract (LRT) and the fungi in the domestic environment. Samples studied consisted of Broncho-Alveolar Lavage (BAL) from 45 patients who underwent bronchoscopy for different diagnostic purposes, and dust and air from the houses (ENV) of 20 of them (44.4%). Additionally, five bronchoscopes (BS) were also analyzed and negative controls were included for every procedure. All samples were processed for DNA extraction and cultures, which were performed in Sabouraud Dextrose and Potato Dextrose Agar. The fungal Internal Transcribed Spacer (ITS2) was sequenced by the Solexa/Illumina system and sequences were analyzed by QIIME 1.8.0 and compared with the UNITE Database for identification. The similarity between the two fungal communities (BAL and ENV) for a specific patient was assessed via the percentage of coincidence in the detection of specific operational taxonomic units (OTUs), and about 75% of co-occurrence was detected between the mycobiome of the LRT and the houses. Cultures confirmed the presence of the core mycobiome species. However, the low rate of isolation from BAL suggests that most of its mycobiome corresponds to non-culturable cells. This likely depends on the patient's immune system activity and inflammatory status.
Filiaciones:
Rubio-Portillo E:
Department of Physiology, Genetics and Microbiology, University of Alicante, Sant Vicent del Raspeig, 03690 Alicante, Spain
Orts D:
Pneumology Service, General University Hospital, Elda, 03600 Alicante, Spain
Llorca E:
Pneumology Service, General University Hospital, Elda, 03600 Alicante, Spain
Fernández C:
Respiratory Department, General University Hospital, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), 03010 Alicante, Spain
Antón J:
Department of Physiology, Genetics and Microbiology, University of Alicante, Sant Vicent del Raspeig, 03690 Alicante, Spain
Ferrer C:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
:
Pneumology Department, Vinalopó University Hospital, Elche 03293 Alicante, Spain
Esteban V:
Respiratory Department, University Hospital of Valencia, 46010 Valencia, Spain
Revelles E:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
Pérez-Martín C:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
Gómez-Imbernón E:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
Adsuar J:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
Piqueras P:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
:
Pneumology Department, Vinalopó University Hospital, Elche 03293 Alicante, Spain
Franco J:
Respiratory Department, University Hospital of Valencia, 46010 Valencia, Spain
Colom MF:
Medical Mycology Laboratory, Department of Plant Production and Microbiology, Institute for Healthcare and Biomedical Research of Alicante (ISABIAL), Campus of Sant Joan d'Alacant, University Miguel Hernández, 03550 Alicante, Spain
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