Genomic Epidemiology ofSalmonellaInfantis in Ecuador: From Poultry Farms to Human Infections
Por:
Mejía L, Medina JL, Bayas R, Salazar CS, Villavicencio F, Zapata S, Matheu J, Wagenaar JA, González-Candelas F and Vinueza-Burgos C
Publicada:
29 sep 2020
Ahead of Print:
29 sep 2020
Categoría:
Veterinary (miscellaneous)
Resumen:
Salmonella entericais one of the most important foodborne pathogens around the world. In the last years,S.entericaserovar Infantis has become an important emerging pathogen in many countries, often as multidrug resistant clones. To understand the importance ofS. entericain the broiler industry in Ecuador, we performed a study based on phenotypic and WGS data of isolates from poultry farms, chicken carcasses and humans. We showed a high prevalence ofS. entericain poultry farms (41.4%) and chicken carcasses (55.5%), but a low prevalence (1.98%) in human samples.S. Infantis was shown to be the most prevalent serovar with a 98.2, 97.8, and 50% in farms, foods, and humans, respectively, presenting multidrug resistant patterns. All sequencedS. Infantis isolates belonged to ST32. For the first time, a pESI-related megaplasmid was identified in Ecuadorian samples. This plasmid contains genes of antimicrobial resistance, virulence factors, and environmental stress tolerance. Genomic analysis showed a low divergence ofS. Infantis strains in the three analyzed components. The results from this study provide important information about genetic elements that may help understand the molecular epidemiology ofS. Infantis in Ecuador.
Filiaciones:
:
Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador
Institute for Integrative Systems Biology, University of Valencia, Valencia, Spain
Medina JL:
Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria, Universidad Central del Ecuador, Quito, Ecuador
Bayas R:
Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador
Salazar CS:
Centro de Referencia Nacional de Resistencia a los Antimicrobianos, Instituto Nacional de Investigación en Salud Pública "Leopoldo Izquieta Pérez", Quito, Ecuador
Villavicencio F:
Centro de Referencia Nacional de Resistencia a los Antimicrobianos, Instituto Nacional de Investigación en Salud Pública "Leopoldo Izquieta Pérez", Quito, Ecuador
Zapata S:
Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador
Matheu J:
Department of Food Safety and Zoonoses, World Health Organization (WHO), Geneva, Switzerland
Wagenaar JA:
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands
Wageningen Bioveterinary Research (WBVR), Lelystad, Netherlands
:
Institute for Integrative Systems Biology, University of Valencia, Valencia, Spain
Joint Research Unit "Infection and Public Health" FISABIO-University of Valencia, Valencia, Spain
CIBER (Centro de Investigación Biomédica en Red) in Epidemiology and Public Health, Valencia, Spain
Vinueza-Burgos C:
Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria, Universidad Central del Ecuador, Quito, Ecuador
Green Published, gold
|