Human milk microbiota in sub-acute lactational mastitis induces inflammation and undergoes changes in composition, diversity and load
Por:
Boix-Amorós A, Hernández-Aguilar MT, Artacho A, Collado MC and Mira A
Publicada:
28 oct 2020
Ahead of Print:
28 oct 2020
Categoría:
Multidisciplinary
Resumen:
Sub-acute mastitis (SAM) is a prevalent disease among lactating women, being one of the main reasons for early weaning. Although the etiology and diagnosis of acute mastitis (AM) is well established, little is known about the underlying mechanisms causing SAM. We collected human milk samples from healthy and SAM-suffering mothers, during the course of mastitis and after symptoms disappeared. Total (DNA-based) and active (RNA-based) microbiota were analysed by 16S rRNA gene sequencing and qPCR. Furthermore, mammary epithelial cell lines were exposed to milk pellets, and levels of the pro-inflammatory interleukin IL8 were measured. Bacterial load was significantly higher in the mastitis samples and decreased after clinical symptoms disappeared. Bacterial diversity was lower in SAM milk samples, and differences in bacterial composition and activity were also found. Contrary to AM, the same bacterial species were found in samples from healthy and SAM mothers, although at different proportions, indicating a dysbiotic ecological shift. Finally, mammary epithelial cell exposure to SAM milk pellets showed an over-production of IL8. Our work therefore supports that SAM has a bacterial origin, with increased bacterial loads, reduced diversity and altered composition, which partly recovered after treatment, suggesting a polymicrobial and variable etiology.
Filiaciones:
:
Department of Biotechnology, Spanish National Research Council (IATA-CSIC), Institute of Agrochemistry and Food Technology, Paterna, Spain
Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 1470 Madison Avenue, New York, NY, 10029, USA
:
Dr Peset Lactation Unit, National Health Service, Dr. Peset University Hospital, Valencia, Spain
:
Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain
Collado MC:
Department of Biotechnology, Spanish National Research Council (IATA-CSIC), Institute of Agrochemistry and Food Technology, Paterna, Spain
:
Department of Biotechnology, Spanish National Research Council (IATA-CSIC), Institute of Agrochemistry and Food Technology, Paterna, Spain.
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