Blood Bacterial Profiles Associated With Human Immunodeficiency Virus Infection and Immune Recovery
Por:
Serrano-Villar S, Sanchez-Carrillo S, Talavera-Rodríguez A, Lelouvier B, Gutiérrez C, Vallejo A, Servant F, Bernadino JI, Estrada V, Madrid N, Gosalbes MJ, Bisbal O, de Lagarde M, Martínez-Sanz J, Ron R, Herrera S, Moreno S and Ferrer M
Publicada:
1 feb 2021
Ahead of Print:
30 jun 2020
Resumen:
Human immunodeficiency virus (HIV) infection impairs mucosal immunity and leads to bacterial translocation, fueling chronic inflammation and disease progression. While this is well established, questions remain about the compositional profile of the translocated bacteria, and to what extent it is influenced by antiretroviral therapy (ART). Using 16S ribosomal DNA targeted sequencing and shotgun proteomics, we showed that HIV increases bacterial translocation from the gut to the blood. HIV increased alpha diversity in the blood, which was dominated by aerobic bacteria belonging to Micrococcaceae (Actinobacteria) and Pseudomonadaceae (Proteobacteria) families, and the number of circulating bacterial proteins was also increased. Forty-eight weeks of ART attenuated this phenomenon. We found that enrichment with Lactobacillales order, and depletion of Actinobacteria class and Moraxellaceae and Corynebacteriacae families, were significantly associated with greater immune recovery and correlated with several inflammatory markers. Our findings suggest that the molecular cross talk between the host and the translocated bacterial products could influence ART-mediated immune recovery.
Filiaciones:
Serrano-Villar S:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Sanchez-Carrillo S:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
Talavera-Rodríguez A:
Bioinformatics Unit, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Lelouvier B:
Vaiomer SAS, Labège, France
Gutiérrez C:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Vallejo A:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Servant F:
Vaiomer SAS, Labège, France
Bernadino JI:
HIV Unit, Hospital Universitario La Paz, Madrid, Spain
Estrada V:
HIV Unit, Hospital Clínico San Carlos, Madrid, Spain
Madrid N:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
:
Area of Genomics and Health, FISABIO-Salud Pública, Valencia, Spain
CIBER Epidemiología y Salud Pública, Madrid, Spain
Bisbal O:
HIV Unit, Hospital Universitario Doce de Octubre, Madrid, Spain
de Lagarde M:
HIV Unit, Hospital Universitario Doce de Octubre, Madrid, Spain
Martínez-Sanz J:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Ron R:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Herrera S:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Moreno S:
Department of Infectious Diseases, Hospital Universitario Ramón y Cajal, Facultad de Medicina, Universidad de Alcalá, IRYCIS, Madrid, Spain
Ferrer M:
Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain
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