Advanced strategy to exploit wine-making waste by manufacturing antioxidant and prebiotic fibre-enriched vesicles for intestinal health
Por:
Allaw M, Manca ML, Caddeo C, Recio MC, Pérez-Brocal V, Moya A, Fernàndez-Busquets X and Manconi M
Publicada:
1 sep 2020
Ahead of Print:
24 may 2020
Resumen:
Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose (R) FM06). The nutriosomes were small in size (from similar to 140 to 260 nm), homogeneous (polydispersity index < 0.2) and highly negative (similar to -79 mV). The vesicles were highly stable during 12 months of storage at 25 degrees C. When diluted with warmed (37 degrees C) acidic medium (pH 1.2) of high ionic strength, the vesicles only displayed an increase of the mean diameter and a low release of the extract, which were dependent on Nutriose concentration. The formulations were highly bio-compatible and able to protect intestinal cells (Caco-2) from oxidative stress damage. In vivo results underlined that the composition of mouse microbiota was not affected by the vesicular formulations. Overall results support the potential application of grape nutriosomes as an alternative strategy for the protection of the intestinal tract.
Filiaciones:
Allaw M:
Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy
Manca ML:
Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy
Caddeo C:
Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy
Recio MC:
Dept. of Pharmacology, Faculty of Pharmacy, University of Valencia, Avda Vicent Andrés Estellés sn, 46100 Burjassot-Valencia, Spain
:
Dept. of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
:
Dept. of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG), València, Spain
Fernàndez-Busquets X:
Institute for Bioengineering of Catalonia, (IBEC), The Barcelona Institute of Science and Technology, Barcelona, Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Manconi M:
Dept. of Scienze della Vita e dell'Ambiente, Sezione di Scienze del Farmaco, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy
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