Caenorhabditis elegans as an in vivo model to assess fucoidan bioactivity preventing Helicobacter pylori infection
Por:
Palacios-Gorba C, Pina R, Tortajada-Girbés M, Jiménez-Belenguer A, Siguemoto É, Ferrús MA, Rodrigo D and Pina-Pérez MC
Publicada:
1 may 2020
Ahead of Print:
12 may 2020
Resumen:
Currently, Helicobacter pylori is the unique biological carcinogenic agent. The search for antimicrobial alternatives to antibiotics against this pathogen has been categorized as a priority due to the drastic failure associated with current applied antibiotic therapy. The present study assessed the bioactive antimicrobial capability of fucoidan ("Generally Recognized as Safe" approval - European Commission December 2017) from different species of Phaeophyceae algae (Fucus vesiculosus, Undaria pinnatifida, Macrocystis pyrifera) against H. pylori. All the studied fucoidans showed bacteriostatic and bactericidal effects at the studied concentrations [5-100] mu g ml(-1) and exposure times [0-7 days]. The most effective anti-H. pylori fucoidan was validated in Caenorhabditis elegans as an in vivo model. C. elegans feed was supplemented with Undaria pinnatifida [0-100] mu g ml(-1) fucoidan, resulting in a significant improvement in lifespan, lowered H. pylori concentration in the digestive tract, and increased egg-laying pattern. New research lines proposing this compound as an active agent in nutraceutical and preventive novel therapies should be opened.
Filiaciones:
Palacios-Gorba C:
Universidad Cardenal Herrera-CEU, Facultad de Veterinaria, Avenida Seminario s/n, 46113 Moncada, Valencia, Spain
Pina R:
Hospital Universitario y Politécnico La Fe, Avenida de Fernando Abril Martorell, 106, 46026, Valencia, Spain
:
Hospital Universitario Doctor Peset, Av. de Gaspar Aguilar, 90, 46017 Valencia, Spain
Jiménez-Belenguer A:
Departamento Biotecnología, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain
Siguemoto É:
University of São Paulo, USP, Av. Prof. Lineu Prestes, Sao Polo, Brazil
Ferrús MA:
Departamento Biotecnología, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain
Rodrigo D:
Institute of Agrochemistry and Food Technology, (IATA-CSIC), Avda. Agustín Escardino, 7, 46022, Paterna, Valencia, Spain
Pina-Pérez MC:
Universidad de Valencia, Departamento Microbiologia y Ecologia, Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain.
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