Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile
Por:
Arnoriaga-Rodríguez M, Mayneris-Perxachs J, Burokas A, Pérez-Brocal V, Moya A, Portero-Otin M, Ricart W, Maldonado R and Fernández-Real JM
Publicada:
30 abr 2020
Ahead of Print:
30 abr 2020
Resumen:
Background The chaperone ClpB, a bacterial protein, is a conformational antigen-mimetic of alpha-melanocyte-stimulating hormone (alpha-MSH) implicated in body weight regulation in mice. We here investigated the potential associations of gut bacterial ClpB-like gene function with obesity status and gut microbiota in humans. Results Gut microbiota ClpB KEGG function was negatively associated with body mass index, waist circumference, and total fat mass (DEXA). The relative abundance (RA) of several phyla and families directly associated with ClpB was decreased in subjects with obesity. Specifically, the RA of Rikenellaceae, Clostridiaceae and not assigned Firmicutes were lower in subjects with obesity and positively associated with gut bacterial ClpB-like gene function (not assigned Firmicutes (r = 0.405, FDR = 2.93 x 10-2), Rikenellaceae (r = 0.217, FDR = 0.031), and Clostridiaceae (r = 0.239, FDR = 0.017)). The gut bacterial ClpB-like gene function was also linked to specific plasma metabolites (hippuric acid and 3-indolepropionic acid) and fecal lupeol. The alpha-MSH-like epitope similar to that of Escherichia coli ClpB was also identified in some sequences of those bacterial families. After fecal transplantation from humans to mice, the families that more contributed to ClpB-like gene function in humans were also associated with ClpB-like gene function in mice after adjusting for the donor's body mass index (not assigned Firmicutes (r = 0.621, p = 0.003), Prevotellaceae (r = 0.725, p = 4.1 x 10(-7)), Rikenellaceae (r = 0.702, p = 3.9 x 10(-4)), and Ruminococcaceae (r = 0.526, p = 0.014)). Clostridiaceae (r = - 0.445, p = 0.038) and Prevotellaceae RA (r = - 0.479, p = 0.024) and were also negatively associated with weight gain in mice. The absolute abundance (AA) of Prevotellaceae in mice was also positively associated with the gut bacterial ClpB-like gene function in mice. DESeq2 identified species of Prevotellaceae, both negatively associated with mice' weight gain and positively with gut bacterial ClpB-like gene function. Conclusions In summary, gut bacterial ClpB-like gene function is associated with obesity status, a specific gut microbiota composition and a plasma metabolomics profile in humans that could be partially transplanted to mice.
Filiaciones:
Arnoriaga-Rodríguez M:
Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Carretera de França s/n, 17007, Girona, Spain
Nutrition, Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain
Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain
Mayneris-Perxachs J:
Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Carretera de França s/n, 17007, Girona, Spain
Nutrition, Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain
Burokas A:
Laboratory of Neuropharmacology, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain
Present address: Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257, Vilnius, Lithuania
:
Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
Biomedical Research Networking Center for Epidemiology and Public Health (CIBERESP), Madrid, Spain
:
Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
Biomedical Research Networking Center for Epidemiology and Public Health (CIBERESP), Madrid, Spain
Institute for Integrative Systems Biology (I2SysBio), University of Valencia, Spanish National Research Council (CSIC-UVEG), Valencia, Spain
Portero-Otin M:
Metabolic Pathophysiology Research Group, Lleida Biomedical Research Institute (IRBLleida), Universitat de Lleida, Lleida, Spain
Ricart W:
Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Carretera de França s/n, 17007, Girona, Spain
Nutrition, Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain
Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain
Maldonado R:
Laboratory of Neuropharmacology, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain
Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain
Fernández-Real JM:
Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Carretera de França s/n, 17007, Girona, Spain.
Nutrition, Eumetabolism and Health Group, Girona Biomedical Research Institute (IdibGi), Girona, Spain.
CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Madrid, Spain.
Department of Medical Sciences, Faculty of Medicine, University of Girona, Girona, Spain.
Green Published, gold
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