Aequorin-expressing yeast emits light under electric control
Por:
Vilanova, C, Hueso, A, Palanca, C, Marco, G, Pitarch, M, Otero, E, Crespo, J, Szablowski, J, Rivera, S, Domínguez-Escribà, L, Navarro, E, Montagud, A, Fernández de Córdoba, P, González, A, Ariño, J, Moya, A, Urchueguía, J and Porcar, M
Publicada:
20 mar 2011
Resumen:
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5 V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control. (C) 2011 Elsevier B.V. All rights reserved.
Green Published
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