Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain
Por:
Domingo-Calap P, Beamud B, Vienne J, González-Candelas F and Sanjuán R
Publicada:
2 ene 2020
Resumen:
The emergence of multi-drug-resistant bacteria represents a major public-health threat. Phages constitute a promising alternative to chemical antibiotics due to their high host specificity, abundance in nature, and evolvability. However, phage host specificity means that highly diverse bacterial species are particularly difficult to target for phage therapy. This is the case of Klebsiella pneumoniae, which presents a hypervariable extracellular matrix capsule exhibiting dozens of variants. Here, we report four novel phages infecting K. pneumoniae capsular type K22 which were isolated from environmental samples in Valencia, Spain. Full genome sequencing showed that these phages belong to the Podoviridae family and encode putative depolymerases that allow digestion of specific K22 K. pneumoniae capsules. Our results confirm the capsular type-specificity of K. pneumoniae phages, as indicated by their narrow infectivity in a panel of K. pneumoniae clinical isolates. Nonetheless, this work represents a step forward in the characterization of phage diversity, which may culminate in the future use of large panels of phages for typing and/or for combating multi-drug-resistant K. pneumoniae.
Filiaciones:
Domingo-Calap P:
Department of Genetics, Institute for Integrative Systems Biology, I2SysBio, Universitat de València, Universitat de València-CSIC, 46980 Paterna, Spain
:
Institute for Integrative Systems Biology, I2SysBio, FISABIO-Salud Pública, Generalitat Valenciana, Universitat de València-CSIC, 46980 Paterna, Spain
Vienne J:
College West Flanders, Howest University, 8500 Kortrijk, Belgium
:
FISABIO-Salud Pública, Generalitat Valenciana, and CIBER in Epidemiology and Public Health, Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980 Paterna, Spain
Sanjuán R:
Institute for Integrative Systems Biology, I2SysBio, Universitat de València-CSIC, 46980 Paterna, Spain
gold, Green Published
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