AMPK modulatory activity of olive-tree leaves phenolic compounds: Bioassay-guided isolation on adipocyte model and in silico approach
Por:
Jiménez-Sánchez C, Olivares-Vicente M, Rodríguez-Pérez C, Herranz-López M, Lozano-Sánchez J, Segura-Carretero A, Fernández-Gutiérrez A, Encinar JA and Micol V
Publicada:
9 mar 2017
Resumen:
Scope
Olive-tree polyphenols have demonstrated potential for the management of obesity-related pathologies. We aimed to explore the capacity of Olive-tree leaves extract to modulate triglyceride accumulation and AMP-activated protein kinase activity (AMPK) on a hypertrophic adipocyte model.
Methods
Intracellular triglycerides and AMPK activity were measured on the hypertrophic 3T3-L1 adipocyte model by AdipoRed and immunofluorescence microscopy, respectively. Reverse phase high performance liquid chromatography coupled to time-of-flight mass detection with electrospray ionization (RP-HPLC-ESI-TOF/MS) was used for the fractionation of the extract and the identification of the compounds. In-silico molecular docking of the AMPK alpha-2, beta and gamma subunits with the identified compounds was performed.
Results
Olive-tree leaves extract decreased the intracellular lipid accumulation through AMPK-dependent mechanisms in hypertrophic adipocytes. Secoiridoids, cinnamic acids, phenylethanoids and phenylpropanoids, flavonoids and lignans were the candidates predicted to account for this effect. Molecular docking revealed that some compounds may be AMPK-gamma modulators. The modulatory effects of compounds over the alpha and beta AMPK subunits appear to be less probable.
Conclusions
Olive-tree leaves polyphenols modulate AMPK activity, which may become a therapeutic aid in the management of obesity-associated disturbances. The natural occurrence of these compounds may have important nutritional implications for the design of functional ingredients.
Filiaciones:
Jiménez-Sánchez C:
Department of Analytical Chemistry, University of Granada. Granada, Spain
Research and Development of Functional Food Centre (CIDAF), PTS, Granada, Spain
Olivares-Vicente M:
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University (UMH), Elche, Alicante, Spain
Rodríguez-Pérez C:
Department of Analytical Chemistry, University of Granada. Granada, Spain
Research and Development of Functional Food Centre (CIDAF), PTS, Granada, Spain
Herranz-López M:
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University (UMH), Elche, Alicante, Spain
Lozano-Sánchez J:
Department of Analytical Chemistry, University of Granada. Granada, Spain
Research and Development of Functional Food Centre (CIDAF), PTS, Granada, Spain
Segura-Carretero A:
Department of Analytical Chemistry, University of Granada. Granada, Spain
Research and Development of Functional Food Centre (CIDAF), PTS, Granada, Spain
Fernández-Gutiérrez A:
Department of Analytical Chemistry, University of Granada. Granada, Spain
Research and Development of Functional Food Centre (CIDAF), PTS, Granada, Spain
:
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University (UMH), Elche, Alicante, Spain
Micol V:
Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University (UMH), Elche, Alicante, Spain
CIBER: CB12/03/30038, Fisiopatología de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III (ISCIII), Palma de Mallorca, Spain
Green Submitted, Green Published, gold
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