Determination of Anti-Anisakis Simplex Antibodies and Relationship with alpha beta and gamma delta Lymphocyte Subpopulations in Patients with Crohn's Disease
Por:
Benet-Campos C, Cuéllar C, García-Ballesteros C, Zamora V, Gil-Borrás R, Catalán-Serra I, López-Chuliá F and Andreu-Ballester JC
Publicada:
1 abr 2017
Resumen:
The etiology of Crohn's disease (CD) is still unknown although new theories are based on defects in innate immunity. We have previously shown a decrease in gamma delta T cells in CD patients. Previous studies have shown a high prevalence of anti-A. simplex immunoglobulins in CD patients. The diminution of gamma delta T cells in the peripheral blood and intestinal mucosa of CD patients may create a state of immunosuppression that would facilitate A. simplex infection.
To study the antibody responses to Anisakis antigens in Crohn's disease patients and its relationship with alpha beta and gamma delta T cell subsets.
We recruited 81 CD patients and 81 healthy controls. alpha beta and gamma delta T cell subsets and anti-A. simplex antibodies were measured.
Levels of anti-A. simplex IgG and IgM were significantly increased in CD patients. Almost 20% of CD patients were positive for IgG and IgM anti-A. simplex versus only 3.7 and 2.5%, respectively, in normal subjects. However, lower specific IgA levels were observed in the group of CD patients versus healthy subjects. We found an association between CD3 + CD8 + gamma delta subset and IgM anti-A. simplex levels. In ileal cases and stricturing behavior of CD, we observed the highest levels of specific antibodies with the exception of anti-A. simplex IgA.
The relationship of specific antibodies with a gamma delta T cell deficiency makes these cell candidates to play a role in the immune response against Anisakis. In addition, anti-Anisakis antibodies could be considered as markers of risk of progression in CD.
Filiaciones:
:
Hematology Department, Arnau de Vilanova Hospital, Valencia, Spain
Cuéllar C:
Department of Parasitology, Faculty of Pharmacy, Complutense University, Madrid, Spain
:
Hematology Department, Arnau de Vilanova Hospital, Valencia, Spain
Zamora V:
Department of Parasitology, Faculty of Pharmacy, Complutense University, Madrid, Spain
Gil-Borrás R:
Digestive Department, IBD Unit, Arnau de Vilanova Hospital, Valencia, Spain
Catalán-Serra I:
Digestive Department, IBD Unit, Arnau de Vilanova Hospital, Valencia, Spain
Internal Medicine, Gastroenterology Department, Levanger Hospital, Helse Nord-Trondelag, Levanger, Norway
Centre of Molecular Inflammation Research (CEMIR), Norwegian Science and Technology University (NTNU), Trondheim, Norway
:
Hematology Department, Arnau de Vilanova Hospital, Valencia, Spain
:
Research Department, Arnau de Vilanova Hospital, c/San Clemente 12, 46015, Valencia, Spain.
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