Neuroprotective and Anti-Inflammatory Effects of a Hydrophilic Saffron Extract in a Model of Glaucoma
Por:
Fernández-Albarral JA, Ramírez AI, de Hoz R, López-Villarín N, Salobrar-García E, López-Cuenca I, Licastro E, Inarejos-García AM, Almodóvar P, Pinazo-Durán MD, Ramírez JM and Salazar JJ
Publicada:
1 sep 2019
Resumen:
Glaucoma is a neurodegenerative disease characterized by the loss of retinal ganglion cells (RGCs). An increase in the intraocular pressure is the principal risk factor for such loss, but controlling this pressure does not always prevent glaucomatous damage. Activation of immune cells resident in the retina (microglia) may contribute to RGC death. Thus, a substance with anti-inflammatory activity may protect against RGC degeneration. This study investigated the neuroprotective and anti-inflammatory effects of a hydrophilic saffron extract standardized to 3% crocin content in a mouse model of unilateral, laser-induced ocular hypertension (OHT). Treatment with saffron extract decreased microglion numbers and morphological signs of their activation, including soma size and process retraction, both in OHT and in contralateral eyes. Saffron extract treatment also partially reversed OHT-induced down-regulation of P2RY12. In addition, the extract prevented retinal ganglion cell death in OHT eyes. Oral administration of saffron extract was able to decrease the neuroinflammation associated with increased intraocular pressure, preventing retinal ganglion cell death. Our findings indicate that saffron extract may exert a protective effect in glaucomatous pathology.
Filiaciones:
Fernández-Albarral JA:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Ramírez AI:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Departamento de Inmunología, Oftalmología y ORL, Facultad de Óptica y Optometría, UCM, 28037 Madrid, Spain
de Hoz R:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Departamento de Inmunología, Oftalmología y ORL, Facultad de Óptica y Optometría, UCM, 28037 Madrid, Spain
López-Villarín N:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Salobrar-García E:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
López-Cuenca I:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Licastro E:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain
Inarejos-García AM:
Pharmactive Biotech Products, SL. Parque Científico de Madrid, Faraday 7, 28049 Madrid, Spain
Almodóvar P:
Pharmactive Biotech Products, SL. Parque Científico de Madrid, Faraday 7, 28049 Madrid, Spain
:
Unidad de Investigación Oftalmológica Santiago Grisolia, Universidad de Valencia, 46017 Valencia, Spain
Ramírez JM:
Instituto de Investigaciones Oftalmológicas Ramón Castroviejo, UCM, 28040 Madrid, Spain.
Departamento de Inmunología, Oftalmología y ORL, Facultad de Medicina, UCM, 28040 Madrid, Spain.
Salazar JJ:
Departamento de Inmunología, Oftalmología y ORL, Facultad de Óptica y Optometría, UCM, 28037 Madrid, Spain.
Green Published, gold, Green Accepted, Green Submitted
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