The Mechanism of the Interactions of Pironetin Analog/Combretastatin A-4 Hybrids with Tubulin.
Por:
Torijano-Gutiérrez S, Vilanova C, Díaz-Oltra S, Murga J, Falomir E, Carda M, Redondo-Horcajo M, Díaz JF, Barasoain I and Marco JA
Publicada:
1 ago 2015
Resumen:
We here report an investigation of the interactions with tubulin of two types of molecules of a hybrid structural type consisting in a combretastatin A-4 moiety and a simplified pironetin fragment. The cytotoxicities of the molecules on two reference tumoral cell lines were measured. In addition, the effects of the compounds on the cell cycle and on microtubule assembly were observed. The dynamics of microtubule polymerization was investigated by means of immunofluorescence assays. It was thus established that at least some of the compounds under study exert their cytotoxic action by means of interaction with tubulin.
Filiaciones:
Torijano-Gutiérrez S:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
Vilanova C:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
Díaz-Oltra S:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
Murga J:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
Carda M:
Departamento de Química Inorgánica y Orgánica, Universidad Jaume I, Castellón, Spain
Redondo-Horcajo M:
CIB, CSIC, Madrid, Spain
Díaz JF:
CIB, CSIC, Madrid, Spain
Barasoain I:
CIB, CSIC, Madrid, Spain
Marco JA:
Departamento de Química Orgánica, Universidad Valencia, Burjassot, Spain
|