Fabrication by Laser Irradiation in a Continuous Flow Jet of Carbon Quantum Dots for Fluorescence Imaging
Por:
Doñate-Buendia C, Torres-Mendieta R, Pyatenko A, Falomir E, Fernández-Alonso M and Mínguez-Vega G
Publicada:
1 mar 2018
Resumen:
Fluorescent carbon quantum dots (CQDs) are synthesized by laser irradiation of carbon glassy particles suspended in polyethylene glycol 200 by two methods, a batch and a flow jet configuration. The flow jet configuration is carried out by the simple combination of common laboratory objects to construct a home-made passage reactor of continuous flow. Despite the simplicity of the system, the laser energy is better harvested by the carbon microparticles, improving the fabrication efficiency a 15% and enhancing the fluorescence of CQDs by an order of magnitude in comparison with the conventional batch. The flow jet-synthesized CQDs have a mean size of 3 nm and are used for fluorescent imaging of transparent healthy and cancer epithelial human cells. Complete internalization is observed with a short incubation time of 10 min without using any extra additive or processing of the cell culture. The CQDs are well fixed in the organelles of the cell even after its death; hence, this is a simple manner to keep the cell information for prolonged periods of time. Moreover, the integrated photostability of the CQDs internalized in in vitro cells is measured and it remains almost constant during at least 2 h, revealing their outstanding performance as fluorescent labels.
Filiaciones:
Doñate-Buendia C:
GROC·UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón, Spain
Torres-Mendieta R:
Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 1402/2, 461 17 Liberec, Czech Republic
Pyatenko A:
Nanomaterials Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan
:
Department of Inorganic and Organic Chemistry, University Jaume I, Avda. Sos Baynat sn, 12071 Castellón, Spain
Fernández-Alonso M:
GROC·UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón, Spain
Mínguez-Vega G:
GROC·UJI, Institute of New Imaging Technologies, Universitat Jaume I, Avda. Sos Baynat sn, 12071 Castellón, Spain
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