Induction of Oxidative Stress and Human Leukocyte/Endothelial Cell Interactions in Polycystic Ovary Syndrome Patients with Insulin Resistance
Por:
Victor, V, Rocha, M, Banuls, C, Alvarez, A, de Pablo, C, Sanchez-Serrano, M, Gomez, M and Hernandez-Mijares, A
Publicada:
1 oct 2011
Resumen:
Context: Insulin resistance is a feature of polycystic ovary syndrome (PCOS) and is related to mitochondrial and endothelial function.
Objective: We tested whether hyperandrogenic insulin-resistant women with PCOS, who have an increased risk of vascular disease, display impaired leukocyte-endothelium interactions, and mitochondrial dysfunction.
Design and Setting: This was a prospective controlled study conducted in an academic medical center.
Patients: The study population consisted of 43 lean reproductive-age women with PCOS and 39 controls subjects.
Main Outcome Measures: We evaluated anthropometric and metabolic parameters, adhesion molecules, and interactions between leukocytes and human umbilical vein endothelial cells. Mitochondrial function was studied by assessing mitochondrial oxygen consumption, membrane potential, reactive oxygen species production, glutathione levels (GSH), and the oxidized glutathione (GSSG)/GSH ratio in polymorphonuclear cells.
Results: Impairment of mitochondrial function was observed in the PCOS patients, evident in a decrease in oxygen consumption, an increase in reactive oxygen species production, a decrease in the GSH/GSSG ratio and GSH levels, and an undermining of the membrane potential. PCOS was related to a decrease in polymorphonuclear cell rolling velocity and an increase in rolling flux and adhesion. Increases in IL-6 and TNF alpha and adhesion molecules (vascular cell adhesion molecule-1 and E-selectin) were also observed.
Conclusion: This study supports the hypothesis of an association between insulin resistance and an impaired endothelial and mitochondrial oxidative metabolism. The evidence obtained shows that the inflammatory state related to insulin resistance in PCOS induces a leukocyte-endothelium interaction. These findings may explain the increased risk of vascular disease in women with PCOS. (J Clin Endocrinol Metab 96: 3115-3122, 2011)
Filiaciones:
:
Hosp Univ Doctor Peset Fdn, Valencia 46017, Spain
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia 46017, Spain
Univ Valencia, Dept Pharmacol, Fac Med, Valencia 46010, Spain
Univ Valencia, Biomed Res Networking Ctr, CIBER Hepat & Digest Dis CB06 04 0071, Fac Med, Valencia 46010, Spain
Univ Valencia, Ctr Invest Biomed Red Hepat & Digest Dis, Fac Med, Valencia 46010, Spain
Univ Valencia, Dept Physiol, Fac Med, Valencia 46010, Spain
:
Hosp Univ Doctor Peset Fdn, Valencia 46017, Spain
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia 46017, Spain
:
Hosp Univ Doctor Peset Fdn, Valencia 46017, Spain
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia 46017, Spain
:
Univ Valencia, Dept Pharmacol, Fac Med, Valencia 46010, Spain
Univ Valencia, Biomed Res Networking Ctr, CIBER Hepat & Digest Dis CB06 04 0071, Fac Med, Valencia 46010, Spain
:
Univ Valencia, Dept Pharmacol, Fac Med, Valencia 46010, Spain
Univ Valencia, Biomed Res Networking Ctr, CIBER Hepat & Digest Dis CB06 04 0071, Fac Med, Valencia 46010, Spain
:
Hosp Univ Doctor Peset Fdn, Valencia 46017, Spain
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia 46017, Spain
:
Hosp Univ Doctor Peset Fdn, Valencia 46017, Spain
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia 46017, Spain
Univ Valencia, Dept Med, Fac Med, Valencia 46010, Spain
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