Human Leukocyte/Endothelial Cell Interactions and Mitochondrial Dysfunction in Type 2 Diabetic Patients and Their Association With Silent Myocardial Ischemia
Por:
Hernandez-Mijares, A, Rocha, M, Rovira-Llopis, S, Banuls, C, Bellod, L, de Pablo, C, Alvarez, A, Roldan-Torres, I, Sola-Izquierdo, E and Victor, V
Publicada:
1 jun 2013
Resumen:
OBJECTIVE-Diabetes is associated with oxidative stress and increased mortality, but a possible correlation between leukocyte-endothelium interactions, oxidative stress, and silent myocardial ischemia (SMI) is yet to be confirmed.
RESEARCH DESIGN AND METHODS-Mitochondrial dysfunction and interactions between leukocytes and human umbilical vein endothelial cells were evaluated in 200 type 2 diabetic patients (25 with SMI) and 60 body composition and age-matched control subjects. A possible correlation between these parameters and the onset of SMI was explored, and anthropometric and metabolic parameters were also analyzed.
RESULTS-Waist, levels of triglycerides, proinflammatory cytokines (interleukin-6 and tumor necrosis factor-alpha, HbA(1c), high-sensitivity C-reactive protein (hs-CRP), glucose, and insulin, and homeostasis model assessment of insulin resistance were higher in diabetic patients than in control subjects. However, no statistical differences in hs-CRP and insulin levels were detected when the data were adjusted for waist. None of these parameters varied between SMI and non-SMI patients. Mitochondrial function was impaired and leukocyte-endothelium interactions were more frequent among diabetic patients, which was evident in the lower mitochondrial 02 consumption, membrane potential, polymorphonuclear cell rolling velocity, and GSH/GSSG ratio, and in the higher mitochondrial reactive oxygen species production and rolling flux, adhesion, and vascular cell adhesion molecule-1 (VCAM-1) and E-selectin molecules observed in these subjects. Moreover, these differences correlated with SMI. Statistical differences were maintained after adjusting the data for BMI and waist, with the exception of VCAM-1 levels when adjusted for waist.
CONCLUSIONS-Oxidative stress, mitochondrial dysfunction, and endothelium-inducing leukocyte-endothelium interactions are features of type 2 diabetes and correlate with SMI.
Filiaciones:
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Univ Valencia, Dept Med, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
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Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
:
Univ Valencia, CIBER Hepat & Digest Dis, Dept Pharmacol, Valencia, Spain
Univ Valencia, CIBER Hepat & Digest Dis, CIBER Res Grp CB061 04 0071, Valencia, Spain
:
Univ Valencia, CIBER Hepat & Digest Dis, Dept Pharmacol, Valencia, Spain
Univ Valencia, CIBER Hepat & Digest Dis, CIBER Res Grp CB061 04 0071, Valencia, Spain
Univ Valencia, Gen Fdn, Valencia, Spain
:
Univ Hosp Doctor Peset, Serv Cardiol, Valencia, Spain
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Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
:
Univ Hosp Doctor Peset, Serv Endocrinol, Valencia, Spain
Fdn Promot Healthcare & Biomed Res Valencian Comm, Valencia, Spain
INCLIVA Fdn, Valencia, Spain
Univ Valencia, CIBER Hepat & Digest Dis, Dept Pharmacol, Valencia, Spain
Univ Valencia, CIBER Hepat & Digest Dis, CIBER Res Grp CB061 04 0071, Valencia, Spain
Univ Valencia, Dept Physiol, Valencia, Spain
Green Published, hybrid
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