Stimulated and unstimulated saliva samples have significantly different bacterial profiles
Por:
Gomar-Vercher S, Simón-Soro A, Montiel-Company JM, Almerich-Silla JM and Mira A
Publicada:
1 jun 2018
Ahead of Print:
1 jun 2018
Resumen:
Epidemiological studies use saliva on a regular basis as a non-invasive and easy-to-take sample, which is assumed to be a microbial representative of the oral cavity ecosystem. However, comparative studies between different kinds of saliva samples normally used in microbial studies are scarce. The aim of the current study was to compare oral microbiota composition between two different saliva samples collected simultaneously: non-stimulated saliva with paper points and stimulated saliva collected after chewing paraffin gum. DNA was extracted from saliva samples of ten individuals, then analyzed by 16S rRNA pyrosequencing to describe bacterial diversity. The results demonstrate significant differences between the microbiota of these two kinds of saliva. Stimulated saliva was found to contain an estimated number of species over three times higher than unstimulated saliva. In addition, bacterial composition at the class and genus level was radically different between both types of samples. When compared to other oral niches, both types of saliva showed some similarity to tongue and buccal mucosa, but they do not correlate at all with the bacterial composition described in supra-or sub-gingival dental plaque, questioning their use in etiological and epidemiological studies of oral diseases of microbial origin.
Filiaciones:
Gomar-Vercher S:
University of Valencia, Stomatology Department, Gascó Oliag 1, Valencia, Spain
:
Department of Genomics and Health, Centre for Advanced Research in Public Health, CSISP-FISABIO, Valencia, Spain
Montiel-Company JM:
University of Valencia, Stomatology Department, Gascó Oliag 1, Valencia, Spain
Almerich-Silla JM:
University of Valencia, Stomatology Department, Gascó Oliag 1, Valencia, Spain
:
Department of Genomics and Health, Centre for Advanced Research in Public Health, CSISP-FISABIO, Valencia, Spain
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