Inhibition of Oral Pathogens Adhesion to Human Gingival Fibroblasts by Wine Polyphenols Alone and in Combination with an Oral Probiotic
Por:
Esteban-Fernández A, Zorraquín-Peña I, Ferrer MD, Mira A, Bartolomé B, González de Llano D and Moreno-Arribas MV
Publicada:
7 mar 2018
Ahead of Print:
21 feb 2018
Resumen:
Several benefits have been described for red wine polyphenols and probiotic strains in the promotion of colonic metabolism and health. On the contrary, knowledge about their role in the management of oral health is still scarce. In this work, the antiadhesive capacity of selected red wine polyphenols and oenological extracts against the oral pathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans in an in vitro model of human gingival fibroblasts has been explored as well as their complementary action with the candidate oral probiotic Streptococcus dentisani. Results highlighted the antiadhesive capacity of caffeic and p-coumaric acids as well as grape seed and red wine oenological extracts. Both, caffeic and p-coumaric acids increased their inhibition potential:against S. mutans adhesion when combined with S. dentisani. Additionally, UHPLC-MS/MS analysis demonstrated the oral metabolism of wine phenolics due to both, cellular and bacterial activity.
Filiaciones:
Esteban-Fernández A:
Instituto de Investigación en Ciencias de la Alimentación (CIAL) , CSIC-UAM , c/Nicolás Cabrera, 9 , 28049 Madrid , Spain
Zorraquín-Peña I:
Instituto de Investigación en Ciencias de la Alimentación (CIAL) , CSIC-UAM , c/Nicolás Cabrera, 9 , 28049 Madrid , Spain
:
Department of Health and Genomics, Center for Advanced Research in Public Health , FISABIO Foundation , 46020 Valencia , Spain
CIBER Epidemiology and Public Health , 28029 Madrid , Spain
:
Department of Health and Genomics, Center for Advanced Research in Public Health , FISABIO Foundation , 46020 Valencia , Spain
CIBER Epidemiology and Public Health , 28029 Madrid , Spain
Bartolomé B:
Instituto de Investigación en Ciencias de la Alimentación (CIAL) , CSIC-UAM , c/Nicolás Cabrera, 9 , 28049 Madrid , Spain
González de Llano D:
Instituto de Investigación en Ciencias de la Alimentación (CIAL) , CSIC-UAM , c/Nicolás Cabrera, 9 , 28049 Madrid , Spain
Moreno-Arribas MV:
Instituto de Investigación en Ciencias de la Alimentación (CIAL) , CSIC-UAM , c/Nicolás Cabrera, 9 , 28049 Madrid , Spain
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