Oxidative stress in the oral cavity is driven by individual-specific bacterial communities
Por:
Džunková M, Martinez-Martinez D, Gardlík R, Behuliak M, Janšáková K, Jiménez N, Vázquez-Castellanos JF, Martí JM, D'Auria G, Bandara HMHN, Latorre A, Celec P and Moya A
Publicada:
27 nov 2018
Ahead of Print:
27 nov 2018
Resumen:
The term "bacterial dysbiosis" is being used quite extensively in metagenomic studies, however, the identification of harmful bacteria often fails due to large overlap between the bacterial species found in healthy volunteers and patients. We hypothesized that the pathogenic oral bacteria are individual-specific and they correlate with oxidative stress markers in saliva which reflect the inflammatory processes in the oral cavity. Temporally direct and lagged correlations between the markers and bacterial taxa were computed individually for 26 volunteers who provided saliva samples during one month (21.2 +/- 2.7 samples/volunteer, 551 samples in total). The volunteers' microbiomes differed significantly by their composition and also by their degree of microbiome temporal variability and oxidative stress markers fluctuation. The results showed that each of the marker-taxa pairs can have negative correlations in some volunteers while positive in others. Streptococcus mutans, which used to be associated with caries before the metagenomics era, had the most prominent correlations with the oxidative stress markers, however, these correlations were not confirmed in all volunteers. The importance of longitudinal samples collections in correlation studies was underlined by simulation of single sample collections in 1000 different combinations which produced contradictory results. In conclusion, the distinct intra-individual correlation patterns suggest that different bacterial consortia might be involved in the oxidative stress induction in each human subject. In the future, decreasing cost of DNA sequencing will allow to analyze multiple samples from each patient, which might help to explore potential diagnostic applications and understand pathogenesis of microbiome-associated oral diseases.
Filiaciones:
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
4Australian Centre for Ecogenomics, The University of Queensland, St Lucia, QLD Australia
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
Gardlík R:
5Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
Behuliak M:
5Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
6Institute of Physiology, Academy of Sciences of the Czech Republic, Praha, Czech Republic
Janšáková K:
5Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
7Institute of Physiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
Martí JM:
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
:
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
8Sequencing and Bioinformatics Service of the Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
Bandara HMHN:
9School of Dentistry, The University of Queensland, Herston, QLD Australia
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
Celec P:
5Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia
:
1Department of Genomics and Health, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO-Public Health), Valencia, Spain
CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain
3Institute for Integrative Systems Biology (I2SysBio), The University of Valencia and The Spanish National Research Council (CSIC)-UVEG, Valencia, Spain
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