Multiparameter Flow Cytometry Identification of Neoplastic Subclones: A New Biomarker in Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma.
Por:
Tarin F, Lopez F, Garcia C, Beneit P, Sarmiento H, Manresa P, Alda O, Villarrubia B, Blanes M, Bernabéu J, Amorós C, Sánchez-Sánchez S, Fernández-Miñano C, De paz F, Verdu J, Marco P and Matutes E
Publicada:
1 ene 2019
Ahead of Print:
14 nov 2018
Resumen:
Multiparameter flow cytometry (MFC)-based clonality assessment is a powerful method of diagnosis and follow-up in monoclonal gammopathy of undetermined significance (MGUS) and multiple myeloma (MM). However, the relevance of intraclonal heterogeneity in immunophenotypic studies remains poorly understood. The main objective of this work was to characterize the different immunophenotypic subclones in MGUS and MM patients and to investigate their correlation with disease stages. An 8-color MFC protocol with 17 markers was used to identify the subclones within the neoplastic compartment of 56 MGUS subjects, 151 newly diagnosed MM patients, 30 MM subjects in complete remission with detectable minimal residual disease, and 36 relapsed/refractory MM patients. Two or more clusters were observed in > 85% of MGUS subjects, 75% of stage I MM patients, and < 15% in stage III. Likewise, a significant correlation between the dominant subclone size, secondary cytogenetic features, and changes in the expression of CD27, CD44, and CD81 was detected. The loss of intraclonal equilibrium may be an important factor related with kinetics and risk of progression not well considered to date in MFC studies. The MFC strategy used in this work can provide useful biomarkers in MGUS and MM.
Filiaciones:
Tarin F:
Department of Hematology, Hospital General Universitario de Alicante, Alicante,
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain,
Lopez F:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Garcia C:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Beneit P:
Department of Hematology, Hospital Universitario San Juan de Alicante, San Juan de Alicante, Spain
Sarmiento H:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Manresa P:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Alda O:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Villarrubia B:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Blanes M:
Department of Hematology, Hospital General Universitario Virgen de la Salud, Elda, Spain
:
Department of Hematology, Hospital General Universitario Virgen de la Salud, Elda, Spain
:
Department of Hematology, Hospital Virgen de los Lirios, Alcoy, Spain
:
Department of Hematology, Hospital Marina Baixa, Villajoyosa, Spain
:
Department of Hematology, Hospital Vega Baja, Orihuela, Spain
De paz F:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Verdu J:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Marco P:
Department of Hematology, Hospital General Universitario de Alicante, Alicante, Spain
Matutes E:
Department of Hematology, Hospital Clinic de Barcelona, Barcelona, Spain
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