Phylogeny of Vibrio vulnificus from the Analysis of the Core-Genome: Implications for Intra-Species Taxonomy
Por:
Roig FJ, González-Candelas F, Sanjuán E, Fouz B, Feil EJ, Llorens C, Baker-Austin C, Oliver JD, Danin-Poleg Y, Gibas CJ, Kashi Y, Gulig PA, Morrison SS and Amaro C
Publicada:
5 ene 2018
Resumen:
Vibrio vulnificus (Vv) is a multi-host pathogenic species currently subdivided into three biotypes (Bts). The three Bts are human-pathogens, but only Bt2 is also a fish-pathogen, an ability that is conferred by a transferable virulence-plasmid (pVvbt2). Here we present a phylogenomic analysis from the core genome of 80 Vv strains belonging to the three Bts recovered from a wide range of geographical and ecological sources. We have identified five well-supported phylogenetic groups or lineages (L). L1 comprises a mixture of clinical and environmental Bt1 strains, most of them involved in human clinical cases related to raw seafood ingestion. L2 is formed by a mixture of Bt1 and Bt2 strains from various sources, including diseased fish, and is related to the aquaculture industry. L3 is also linked to the aquaculture industry and includes Bt3 strains exclusively, mostly related to wound infections or secondary septicemia after farmed-fish handling. Lastly, L4 and L5 include a few strains of Bt1 associated with specific geographical areas. The phylogenetic trees for ChrI and II are not congruent to one another, which suggests that inter-and/or intra-chromosomal rearrangements have been produced along Vv evolution. Further, the phylogenetic trees for each chromosome and the virulence plasmid were also not congruent, which also suggests that pVvbt2 has been acquired independently by different clones, probably in fish farms. From all these clones, the one with zoonotic capabilities (Bt2-Serovar E) has successfully spread worldwide. Based on these results, we propose a new updated classification of the species based on phylogenetic lineages rather than on Bts, as well as the inclusion of all Bt2 strains in a pathovar with the particular ability to cause fish vibriosis, for which we suggest the name "piscis."
Filiaciones:
Roig FJ:
Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain
Departmento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain
Biotechvana, Parc Científic, Universitat de València, Valencia, Spain
:
Joint Research Unit on Infection and Public Health FISABIO-Salud Pública and Universitat de Valencia-I2SysBio, Valencia, Spain
CIBEResp, National Network Center for Research on Epidemiology and Public Health, Instituto de Salud Carlos III, Valencia, Spain
Sanjuán E:
Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain
Departmento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain
Fouz B:
Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain
Departmento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain
Feil EJ:
Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
Llorens C:
Biotechvana, Parc Científic, Universitat de València, Valencia, Spain
Baker-Austin C:
Centre for Environment, Fisheries and Aquaculture Science, Weymouth, United Kingdom
Oliver JD:
Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States
Duke University Marine Lab, Beaufort, NC, United States
Danin-Poleg Y:
Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel
Gibas CJ:
Department of Bioinformatics and Genomics, the University of North Carolina at Charlotte, Charlotte, NC, United States
Kashi Y:
Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel
Gulig PA:
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, United States
Morrison SS:
Department of Bioinformatics and Genomics, the University of North Carolina at Charlotte, Charlotte, NC, United States
Amaro C:
Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain
Departmento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain
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