Obesity impairs leukocyte-endothelium cell interactions and oxidative stress in humans
Por:
Lopez-Domenech S, Banuls C, Diaz-Morales N, Escribano-Lopez I, Morillas C, Veses S, Orden S, Alvarez A, Victor VM, Hernandez-Mijares A and Rocha M
Publicada:
1 ago 2018
Ahead of Print:
20 jun 2018
Resumen:
BackgroundTo evaluate the relationship between leukocyte-endothelial cell interactions and oxidative stress parameters in non-diabetic patients with different grades of obesity.
Material and methodsFor this cross-sectional study, 225 subjects were recruited from January 1, 2014 to December 31, 2016 and divided into groups according to BMI (<30kg/m(2), 30-40kg/m(2) and >40kg/m(2)). We determined clinical parameters, systemic inflammatory markers, soluble cellular adhesion molecules, leukocyte-endothelium cell interactionsrolling flux, velocity and adhesion, oxidative stress parameterstotal ROS, total superoxide, glutathioneand mitochondrial membrane potential in leukocytes.
ResultsWe verified that HOMA-IR and hsCRP increased progressively as obesity developed, whereas A1c, IL6 and TNF were augmented in the BMI>40kg/m(2) group. The cellular adhesion molecule sP-selectin was increased in patients with obesity, while sICAM, total ROS, total superoxide and mitochondrial membrane potential were selectively higher in the BMI>40kg/m(2) group. Obesity induced a progressive decrease in rolling velocity and an enhancement of rolling flux and leukocyte adhesion.
ConclusionOur findings reveal that endothelial dysfunction markers are altered in human obesity and are associated with proinflammatory cytokines and increased oxidative stress parameters.
Filiaciones:
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
Institute of Health Research INCLIVA, University of Valencia, Avda. Menéndez Pelayo 4, 46010, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
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CIBER CB06/04/0071 Research Group. CIBER Hepatic and Digestive Diseases, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
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CIBER CB06/04/0071 Research Group. CIBER Hepatic and Digestive Diseases, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
Universidad Jaume I, Facultad de Ciencias de la Salud, Av. del Dr. Clara, 19, 12002, Castellon de la Plana, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
Institute of Health Research INCLIVA, University of Valencia, Avda. Menéndez Pelayo 4, 46010, Valencia, Spain
CIBER CB06/04/0071 Research Group. CIBER Hepatic and Digestive Diseases, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
Department of Physiology, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
Institute of Health Research INCLIVA, University of Valencia, Avda. Menéndez Pelayo 4, 46010, Valencia, Spain
Department of Medicine, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
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Service of Endocrinology and Nutrition, University Hospital Doctor Peset-FISABIO, Avda. Gaspar Aguilar 90, 46017, Valencia, Spain
Institute of Health Research INCLIVA, University of Valencia, Avda. Menéndez Pelayo 4, 46010, Valencia, Spain
CIBER CB06/04/0071 Research Group. CIBER Hepatic and Digestive Diseases, University of Valencia, Av Blasco Ibanez 13, 46010, Valencia, Spain
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