Gut Microbiota Perturbations in Reactive Arthritis and Postinfectious Spondyloarthritis
Por:
Manasson J, Shen N, Garcia Ferrer HR, Ubeda C, Iraheta I, Heguy A, Von Feldt JM, Espinoza LR, Kutzbach AG, Segal LN, Ogdie A, Clemente JC and Scher JU
Publicada:
1 feb 2018
Ahead of Print:
3 ene 2018
Resumen:
Objective. Reactive arthritis (ReA) is an inflammatory disorder occurring several weeks after gastrointestinal or genitourinary tract infections. HLA-B27 positivity is considered a risk factor, although it is not necessarily predictive of disease incidence. Among nongenetic factors, the intestinal microbiome may play a role in disease susceptibility. The objective of this study was to characterize the gut microbiota and host gene interactions in ReA and postinfectious spondyloarthritis.
Methods. Adult subjects with peripheral spondyloarthritis and control subjects with preceding infections who did not develop arthritis were prospectively recruited from a geographic region with a high prevalence of ReA. Clinical variables, HLA status, and 16S ribosomal RNA gene sequencing of intestinal microbiota were analyzed.
Results. Subjects with ReA showed no significant differences from controls in gut bacterial richness or diversity. However, there was a significantly higher abundance of Erwinia and Pseudomonas and an increased prevalence of typical enteropathogens associated with ReA. Subjects with ultrasound evidence of enthesitis were enriched in Campylobacter, while subjects with uveitis and radiographic sacroiliitis were enriched in Erwinia and unclassified Ruminococcaceae, respectively; both were enriched in Dialister. Host genetics, particularly HLA-A24, were associated with differences in gut microbiota diversity irrespective of disease status. We identified several co-occurring taxa that were also predictive of HLA-A24 status.
Conclusion. This is the first culture-independent study characterizing the gut microbial community in postinfectious arthritis. Although bacterial factors correlated with disease presence and clinical features of ReA, host genetics also appeared to be a major independent driver of intestinal community composition. Understanding of these gut microbiota-host genetic relationships may further clarify the pathogenesis of postinfectious spondyloarthritides.
Filiaciones:
Manasson J:
Department of Medicine, Division of Rheumatology and Psoriatic Arthritis Center, New York University School of Medicine and Hospital for Joint Diseases, New York, NY
Shen N:
Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY
Garcia Ferrer HR:
Guatemalan Association against Rheumatic Diseases (AGAR), Guatemala City, Guatemala
Universidad Francisco Marroquin, Guatemala City, Guatemala
:
Centro Superior de Investigacion en Salud Publica - FISABIO, Valencia, Spain [FISABIO SALUD PÚBLICA - GENÓMICA Y SALUD]
CIBER en Epidemiología y Salud Pública, Madrid, Spain
Iraheta I:
Guatemalan Association against Rheumatic Diseases (AGAR), Guatemala City, Guatemala
Universidad Francisco Marroquin, Guatemala City, Guatemala
Heguy A:
New York University Genome Technology Center, Department of Pathology, New York University School of Medicine, New York, NY
Von Feldt JM:
University of Pennsylvania, Philadelphia, PA, USA
Espinoza LR:
LSU Health Science Center, New Orleans, LA
Kutzbach AG:
Guatemalan Association against Rheumatic Diseases (AGAR), Guatemala City, Guatemala
Universidad Francisco Marroquin, Guatemala City, Guatemala
Segal LN:
Department of Medicine, Division of Pulmonary and Critical Care, New York University School of Medicine, New York, NY
Ogdie A:
University of Pennsylvania, Philadelphia, PA, USA
Clemente JC:
Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY
Scher JU:
Department of Medicine, Division of Rheumatology and Psoriatic Arthritis Center, New York University School of Medicine and Hospital for Joint Diseases, New York, NY
Green Accepted, Bronze
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