A Standardised Approach to the Biomechanical Evaluation of Tracheal Grafts
Por:
Martinez-Hernandez, N, Mas-Estelles, J, Milian-Medina, L, Martinez-Ramos, C, Ceron-Navarro, J, Galbis-Caravajal, J, Roig-Bataller, A and Mata-Roig, M
Publicada:
1 oct 2021
Resumen:
The ideal tracheal substitute must have biomechanical properties comparable to the native trachea, but currently there is no standardised approach to evaluating these properties. Here we propose a novel method for evaluating and comparing the properties of tracheal substitutes, thus systematising both measurement and data curation. This system was tested by comparing native rabbit tracheas to frozen and decellularised specimens and determining the histological characteristics of those specimens. We performed radial compression tests on the anteroposterior tracheal axis and longitudinal axial tensile tests with the specimens anastomosed to the jaw connected to a measuring system. All calculations and results were adjusted according to tracheal size, always using variables relative to the tracheal dimensions, thus permitting comparison of different sized organs. The biomechanical properties of the decellularised specimens were only slightly reduced compared to controls and significant in regard to the maximum stress withstood in the longitudinal axis (-0.246 MPa CI [-0.248, -0.145] MPa) and the energy stored per volume unit (-0.124 mJ & BULL;mm(-3) CI [-0.195, -0.055] mJ & BULL;mm(-3)). The proposed method is suitable for the systematic characterisation of the biomechanical properties of different tracheal substitutes, regardless of the size or nature of the substitute, thus allowing for direct comparisons.
Filiaciones:
:
Hosp Univ de la Ribera, Dept Thorac Surg, Valencia 46600, Spain
Mas-Estelles, J:
Univ Politecn Valencia, Biomat Ctr, Valencia 46022, Spain
:
Univ Valencia, Fac Med & Odontol, Dept Pathol, Valencia 46010, Spain
Martinez-Ramos, C:
Univ Politecn Valencia, Biomat Ctr, Valencia 46022, Spain
Ceron-Navarro, J:
Hosp Univ & Politecn la Fe, Dept Thorac Surg, Valencia 46026, Spain
:
Hosp Univ de la Ribera, Dept Thorac Surg, Valencia 46600, Spain
Roig-Bataller, A:
Hosp Univ de la Ribera, Dept Thorac Surg, Valencia 46600, Spain
Mata-Roig, M:
Univ Valencia, Fac Med & Odontol, Dept Pathol, Valencia 46010, Spain
ISCIII, Networking Res Ctr Resp Dis CIBERER, Madrid 28029, Spain
Green Published, gold
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