Harmonized Quality Assurance/Quality Control Provisions for Nontargeted Measurement of Urinary Pesticide Biomarkers in the HBM4EU Multisite SPECIMEn Study
Por:
Vitale CM, Lommen A, Huber C, Wagner K, Garlito Molina B, Nijssen R, Price EJ, Blokland M, van Tricht F, Mol HGJ, Krauss M, Debrauwer L, Pardo O, Leon N, Klanova J and Antignac JP
Ahead of Print:
1 may 2022
Resumen:
A set of quality assurance/quality control (QA/QC) criteria for nontargeted measurement of pesticide exposure markers in a large-scale study of human urine has been proposed and applied across five laboratories within the HBM4EU project. Quality control material, including reference standards and fortified pooled urine samples (QC urine) were prepared in a centralized way and distributed across participants to monitor analytical performance and consistency of the liquid chromatography coupled to high-resolution mass spectrometry data generated with a harmonized workflow. Signal intensities, mass accuracy, and retention times of selected QA/QC markers covering a broad range of physicochemical properties were monitored across QC solvent standards, QC urine samples, study urine samples, and procedural blanks, setting acceptance thresholds for repeatability and accuracy. Overall, results showed high repeatability of the collected data. The RSDs of the signal intensities were typically below 20-30% in QC and study samples, with good stability of the chromatographic separation (retention time drift within 2-4 s intrabatch and 5 s interbatch) and excellent mass accuracy (average error < 2 ppm). The use of the proposed criteria allowed for the identification of handling errors, instrumental issues, and potential batch effects. This is the first elaboration of harmonized QA/QC criteria applied across multiple laboratories to assess the quality of data generated by nontargeted analysis of human samples.
Filiaciones:
Vitale CM:
RECETOX, Faculty of Science, Masaryk University, Brno 60200, Czech Republic
Lommen A:
Wageningen Food Safety Research, Wageningen University & Research, Wageningen 6708 WB, The Netherlands
Huber C:
Department of Effect-Directed Analysis, Helmholtz Centre for Environmental Research-UFZ, Leipzig 04318, Germany
Institute of Ecology, Diversity and Evolution, Goethe University Frankfurt Biologicum, Campus Riedberg, Frankfurt am Main 60438, Germany
Wagner K:
INRAE, Toxalim, Toulouse 31027, France
:
FISABIO (Foundation for the Promotion of Health and Biomedical Research of the Valencia Region), Valencia 46020, Spain
Nijssen R:
Wageningen Food Safety Research, Wageningen University & Research, Wageningen 6708 WB, The Netherlands
Price EJ:
RECETOX, Faculty of Science, Masaryk University, Brno 60200, Czech Republic
Blokland M:
Wageningen Food Safety Research, Wageningen University & Research, Wageningen 6708 WB, The Netherlands
van Tricht F:
Wageningen Food Safety Research, Wageningen University & Research, Wageningen 6708 WB, The Netherlands
Mol HGJ:
Wageningen Food Safety Research, Wageningen University & Research, Wageningen 6708 WB, The Netherlands
Krauss M:
Department of Effect-Directed Analysis, Helmholtz Centre for Environmental Research-UFZ, Leipzig 04318, Germany
Debrauwer L:
INRAE, Toxalim, Toulouse 31027, France
:
FISABIO (Foundation for the Promotion of Health and Biomedical Research of the Valencia Region), Valencia 46020, Spain
:
FISABIO (Foundation for the Promotion of Health and Biomedical Research of the Valencia Region), Valencia 46020, Spain
Klanova J:
RECETOX, Faculty of Science, Masaryk University, Brno 60200, Czech Republic
Antignac JP:
Oniris, INRAE, LABERCA, Nantes 44300, France
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