Sodium Hyaluronate-Induced Ocular Hypertension in Rats Damages the Direction-Selective Circuit and Inner/Outer Retinal Plexiform Layers
Por:
Noailles A, Kutsyr O, Mayordomo-Febrer A, Lax P, López-Murcia M, Sanz-González SM, Pinazo-Durán MD and Cuenca N
Publicada:
1 may 2022
Resumen:
PURPOSE. To assess the changes in retinal morphology in a rat model of chronic glaucoma induced by ocular hypertension.METHODS. Intraocular pressure (IOP) was surgically increased through weekly injec-tions of sodium hyaluronate (HYA) in the anterior eye chamber of the left eye of male Wistar rats, whereas the right eyes were sham operated (salt solution). During the 10-week experimental period, IOP was measured weekly with a rebound tonometer. Retinal cryosections were prepared for histological/immunohistochemical analysis and morphometry.RESULTS. IOP was higher in HYA-treated eyes than in sham-operated eyes along the 10 -week period, which was significant from the fourth to the nineth week. Ocular hyperten-sion in HYA-treated eyes was associated with morphologic and morphometric changes in bipolar cells, ON-OFF direction-selective ganglion cells, ON/OFF starburst amacrine cells, and inner plexiform layer sublamina. CONCLUSIONS. Serial HYA treatment in the rat anterior eye chamber results in mild-to -moderate elevated and sustained IOP and ganglion cell death, which mimics most human open-angle glaucoma hallmarks. The reduced number of direction-selective ganglion cells and starburst amacrine cells accompanied by a deteriorated ON/OFF plexus in this glaucoma model could lend insight to the abnormalities in motion perception observed in patients with glaucoma.
Filiaciones:
Noailles A:
Physiology, Genetics and Microbiology, University of Alicante, Spain
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Kutsyr O:
Physiology, Genetics and Microbiology, University of Alicante, Spain
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Mayordomo-Febrer A:
Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universidad CEU Cardenal Herrera, Valencia, Spain
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU/FISABIO, Valencia, Spain
Lax P:
Physiology, Genetics and Microbiology, University of Alicante, Spain
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
López-Murcia M:
Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universidad CEU Cardenal Herrera, Valencia, Spain
Mixed Research Unit for Visual Health and Veterinary Ophthalmology CEU/FISABIO, Valencia, Spain
:
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Cellular and Molecular Ophthalmo-biology Research Group, Department of Surgery, University of Valencia, Valencia, Spain
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, Valencia, Spain
:
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Cellular and Molecular Ophthalmo-biology Research Group, Department of Surgery, University of Valencia, Valencia, Spain
Ophthalmic Research Unit "Santiago Grisolía"/FISABIO, Valencia, Spain
Cuenca N:
Physiology, Genetics and Microbiology, University of Alicante, Spain
OFTARED. Spanish Net of Ophthalmic Research. Institute of health Carlos III, Madrid, Spain
Green Published, gold
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