Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA-seq analysis
Por:
Fass, M, Rivarola, M, Ehrenbolger, G, Maringolo, C, Montecchia, J, Quiroz, F, Garcia-Garcia, F, Blazquez, J, Hopp, H, Heinz, R, Paniego, N and Lia, V
Publicada:
7 ago 2020
Categoría:
Multidisciplinary
Resumen:
Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense.
Filiaciones:
Fass, M:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Rivarola, M:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Ehrenbolger, G:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Maringolo, C:
Inst Nacl Tecnol Agr INTA, Estn Expt Agr Balcarce, Balcarce, Argentina
Montecchia, J:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Quiroz, F:
Inst Nacl Tecnol Agr INTA, Estn Expt Agr Balcarce, Balcarce, Argentina
:
Principe Felipe Res Ctr, Bioinfomat & Biostat Unit, Valencia, Spain
Blazquez, J:
Hosp Virgen del Rocio, CDCA, Fdn Progreso & Salud FPS, Clin Bioinformat Area, Seville 41013, Spain
Hosp Virgen del Rocio, FPS, INB ELIXIR Es, Seville 42013, Spain
Hopp, H:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Univ Buenos Aires UBA, Fac Ciencias Exactas & Nat FCEyN, Dept Fisiol Biol Mol & Celular FBMC, 1428 Ciudad Univ, Buenos Aires, DF, Argentina
Heinz, R:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Paniego, N:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
Lia, V:
Consejo Nacl Invest Cient & Tecn, Inst Nacl Tecnol Agr INTA, Inst Agrobiotecnol & Biol Mol IABIMO, Hurlingham B16861GC, Buenos Aires, DF, Argentina
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