Hypoxia Positively Regulates the Expression of pH-Sensing G-Protein-Coupled Receptor OGR1 (GPR68)
Por:
de Valliere, C, Cosin-Roger, J, Simmen, S, Atrott, K, Melhem, H, Zeitz, J, Madanchi, M, Tcymbarevich, I, Fried, M, Kullak-Ublick, G, Vavricka, S, Misselwitz, B, Seuwen, K, Wagner, C, Eloranta, J, Rogler, G and Rui, P
Publicada:
1 nov 2016
Resumen:
BACKGROUND & AIMS: A novel family of proton-sensing G-protein-coupled receptors, including ovarian cancer G-protein-coupled receptor 1 (OGR1) (GPR68) has been identified to play a role in pH homeostasis. Hypoxia is known to change tissue pH as a result of anaerobic glucose metabolism through the stabilization of hypoxia-inducible factor-1 alpha. We investigated how hypoxia regulates the expression of OGR1 in the intestinal mucosa and associated cells.
METHODS: OGR1 expression in murine tumors, human colonic tissue, and myeloid cells was determined by quantitative reverse-transcription polymerase chain reaction. The influence of hypoxia on OGR1 expression was studied in monocytes/macrophages and intestinal mucosa of inflammatory bowel disease (IBD) patients. Changes in OGR1 expression in MonoMac6 (MM6) cells under hypoxia were determined upon stimulation with tumor necrosis factor (TNF), in the presence or absence of nuclear factor-kappa B (NF-kappa B) inhibitors. To study the molecular mechanisms involved, chromatin immunoprecipitation analysis of the OGR1 promoter was performed.
RESULTS: OGR1 expression was significantly higher in tumor tissue compared with normal murine colon tissue. Hypoxia positively regulated the expression of OGR1 in MM6 cells, mouse peritoneal macrophages, primary human intestinal macrophages, and colonic tissue from IBD patients. In MM6 cells, hypoxia-enhanced TNF-induced OGR1 expression was reversed by inhibition of NF-kappa B. In addition to the effect of TNF and hypoxia, OGR1 expression was increased further at low pH. Chromatin immunoprecipitation analysis showed that HIF-1 alpha, but not NF-kappa B, binds to the promoter of OGR1 under hypoxia.
CONCLUSIONS: The enhancement of TNF- and hypoxia-induced OGR1 expression under low pH points to a positive feed-forward regulation of OGR1 activity in acidic conditions, and supports a role for OGR1 in the pathogenesis of IBD.
Filiaciones:
de Valliere, C:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Univ Valencia, Fac Med, Dept Pharmacol, Valencia, Spain
Univ Valencia, Fac Med, Biomed Res Networking Ctr Hepat & Digest Dis CIBE, Valencia, Spain
Simmen, S:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Atrott, K:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Melhem, H:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Zeitz, J:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Madanchi, M:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Tcymbarevich, I:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Fried, M:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Kullak-Ublick, G:
Univ Hosp Zurich, Dept Clin Pharmacol & Toxicol, Zurich, Switzerland
Vavricka, S:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Misselwitz, B:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Seuwen, K:
Novartis Inst Biomed Res, Basel, Switzerland
Wagner, C:
Univ Hosp Zurich, Inst Physiol, Zurich, Switzerland
Eloranta, J:
Univ Hosp Zurich, Dept Clin Pharmacol & Toxicol, Zurich, Switzerland
Rogler, G:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
Rui, P:
Univ Hosp Zurich, Div Gastroenterol & Hepatol, Ramistr 100, CH-8091 Zurich, Switzerland
gold, Green Published
|