Molecular Regulation of the Developing Commissural Plate
Por:
Moldrich, R, Gobius, I, Pollak, T, Zhang, J, Ren, T, Brown, L, Mori, S, De Juan Romero, C, Britanova, O, Tarabykin, V and Richards, L
Publicada:
15 sep 2010
Categoría:
Neuroscience (miscellaneous)
Resumen:
Coordinated transfer of information between the brain hemispheres is essential for function and occurs via three axonal commissures in the,telencephalon: the corpus callosum (CC), hippocampal commissure (HC), and anterior commissure (AC). Commissural malformations occur in over 50 human congenital syndromes causing mild to severe cognitive impairment. Disruption of multiple commissures in some syndromes suggests that common mechanisms may underpin their development. Diffusion tensor magnetic resonance imaging revealed that forebrain commissures crossed the midline in a highly specific manner within an oblique plane of tissue, referred to as the commissural plate. This specific anatomical positioning suggests that correct patterning of the commissural plate may influence forebrain commissure formation. No analysis of the molecular specification of the commissural plate has been performed in any species; therefore, we utilized specific transcription factor markers to delineate the commissural plate and identify its various subdomains. We found that the mouse commissural plate consists of four domains and tested the hypothesis that disruption of these domains might affect commissure formation. Disruption of the dorsal domains occurred in strains with commissural defects such as Emx2 and Nfia knockout mice but commissural plate patterning was normal in other acallosal strains such as Satb2(-/-). Finally, we demonstrate an essential role for the morphogen Fgf8 in establishing the commissural plate at later developmental stages. The results demonstrate that correct patterning of the commissural plate is an important mechanism in forebrain commissure formation. J. Comp. Neurol. 518:3645-3661, 2010. (C) 2010 Wiley-Liss, Inc.
Filiaciones:
Moldrich, R:
Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
Gobius, I:
Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
Pollak, T:
Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
Zhang, J:
Johns Hopkins Univ, Sch Med, Dept Radiol, Div NMR Res, Baltimore, MD 21205 USA
Ren, T:
Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
Brown, L:
Univ Vermont, Dept Obstet, Burlington, VT 05401 USA
Mori, S:
Johns Hopkins Univ, Sch Med, Dept Radiol, Div NMR Res, Baltimore, MD 21205 USA
Kennedy Krieger Inst, FM Kirby Funct Imaging Ctr, Baltimore, MD 21205 USA
:
Max Planck Inst Expt Med, D-37075 Gottingen, Germany
Res Ctr Mol Physiol Brain CMPB, D-37073 Gottingen, Germany
Britanova, O:
Max Planck Inst Expt Med, D-37075 Gottingen, Germany
Res Ctr Mol Physiol Brain CMPB, D-37073 Gottingen, Germany
Tarabykin, V:
Max Planck Inst Expt Med, D-37075 Gottingen, Germany
Res Ctr Mol Physiol Brain CMPB, D-37073 Gottingen, Germany
Richards, L:
Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia
Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia
Green Accepted
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