Discrete domains of gene expression in germinal layers distinguish the development of gyrencephaly
Por:
de Juan Romero, C, Bruder, C, Tomasello, U, Sanz-Anquela, J and Borrell, V
Publicada:
14 jul 2015
Resumen:
Gyrencephalic species develop folds in the cerebral cortex in a stereotypic manner, but the genetic mechanisms underlying this patterning process are unknown. We present a large-scale transcriptomic analysis of individual germinal layers in the developing cortex of the gyrencephalic ferret, comparing between regions prospective of fold and fissure. We find unique transcriptional signatures in each germinal compartment, where thousands of genes are differentially expressed between regions, including similar to 80% of genes mutated in human cortical malformations. These regional differences emerge from the existence of discrete domains of gene expression, which occur at multiple locations across the developing cortex of ferret and human, but not the lissencephalic mouse. Complex expression patterns emerge late during development and map the eventual location of folds or fissures. Protomaps of gene expression within germinal layers may contribute to define cortical folds or functional areas, but our findings demonstrate that they distinguish the development of gyrencephalic cortices.
Filiaciones:
:
CSIC, Inst Neurociencias, Sant Joan dAlacant, Spain
Univ Miguel Hernandez, Sant Joan dAlacant, Spain
Bruder, C:
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden
Tomasello, U:
CSIC, Inst Neurociencias, Sant Joan dAlacant, Spain
Univ Miguel Hernandez, Sant Joan dAlacant, Spain
Sanz-Anquela, J:
Hosp Univ Principe Asturias, Serv Pathol, Alcala De Henares, Spain
Borrell, V:
CSIC, Inst Neurociencias, Sant Joan dAlacant, Spain
Univ Miguel Hernandez, Sant Joan dAlacant, Spain
Green Published, hybrid
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