Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening
Por:
Sentandreu, E, Fuente-Garcia, C, Pardo, O, Olivan, M, Leon, N, Aldai, N, Yusa, V and Sentandreu, M
Publicada:
7 jul 2021
Ahead of Print:
1 jun 2021
Resumen:
An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment.
Filiaciones:
Sentandreu, E:
Inst Agroquim & Tecnologia Alimentos IATA CSIC, Valencia 46980, Spain
Fuente-Garcia, C:
Inst Agroquim & Tecnologia Alimentos IATA CSIC, Valencia 46980, Spain
Univ Basque Country UPV EHU, Dept Pharm & Food Sci, Lactiker Res Grp, Vitoria 01006, Spain
:
Univ Valencia, Analyt Chem Dept, Burjassot 46100, Spain
FISABIO Publ Hlth, Fdn Promot Hlth & Biomed Res Valencia Reg, Valencia 46020, Spain
Olivan, M:
Serv Reg Invest & Desarrollo Alimentario SERIDA, Villaviciosa 33300, Asturias, Spain
:
Publ Hlth Lab Valencia, Valencia 46020, Spain
Aldai, N:
Univ Basque Country UPV EHU, Dept Pharm & Food Sci, Lactiker Res Grp, Vitoria 01006, Spain
:
Publ Hlth Lab Valencia, Valencia 46020, Spain
FISABIO Publ Hlth, Fdn Promot Hlth & Biomed Res Valencia Reg, Valencia 46020, Spain
Univ Valencia, Analyt Chem Dept, Burjassot 46100, Spain
Sentandreu, M:
Inst Agroquim & Tecnologia Alimentos IATA CSIC, Valencia 46980, Spain
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