Intestinal mycobiota composition and changes in children with thalassemia who underwent allogeneic hematopoietic stem cell transplantation
Por:
Yalcin, S, Aksu, T, Kuskonmaz, B, Ozbek, N, Perez-Brocal, V, Celik, M, Cetinkaya, D, Moya, A and Dinleyici, E
Publicada:
1 ene 2022
Ahead of Print:
1 oct 2021
Resumen:
Background: Allogeneic hematopoietic stem cell transplantation (HSCT) alters the diversity of the intestinal bacterial microbiota. This study aimed to evaluate human mycobiota composition pre-HSCT and post-HSCT in children with thalassemia. Method: Ten children with thalassemia undergoing allogeneic HSCT were enrolled. The stool samples were collected before the transplantation regimen, before the transplant day, and +15, +30 days, and three months after transplantation. Stool samples were also collected from the donor and the patient's caregivers. Gut mycobiota composition was evaluated with metagenomic analysis. Results: Pretransplant mycobiota of children with thalassemia (the predominant genus was Saccharomyces, 64.1%) has been shown to approximate the diverse mycobiota compositions of healthy adult donors but becomes altered (lower diversity) following transplant procedures. Three months after HSCT, phyla Ascomycota and Basidiomycota were 83.4% and 15.6%, respectively. The predominant species were Saccaharomyces_uc and Saccharomyces cerevisiae (phylum Ascomycota); we also observed Malassezia restricta and Malassezia globosa (phylum Basidiomycota) (similar to 13%). On day 90 after HSCT, we observed 65.3% M. restricta and 18.4% M. globosa predominance at the species level in a four-year-old boy with acute graft-versus-host disease (GVHD) (skin and gut involvement) 19 days after transplantation included. Conclusion: The mycobiota composition of children with thalassemia altered after HSCT. We observed Malassezia predominance in a child with GVHD. Further studies in children with GVHD will identify this situation.
Filiaciones:
Yalcin, S:
Hacettepe Univ, Fac Med, Dept Social Pediat, Ankara, Turkey
Aksu, T:
Hacettepe Univ, Fac Med, Dept Pediat Hematol, Ankara, Turkey
Kuskonmaz, B:
Hacettepe Univ, Fac Med, Dept Pediat Hematol, Ankara, Turkey
Ozbek, N:
Hlth Sci Univ, Fac Med, Dept Pediat Hematol, Ankara City Hosp, Ankara, Turkey
:
Fdn Promot Sanit & Biomed Res Valencia Reg FISABI, Area Genom & Hlth, Valencia, Spain
CIBER Epidemiol & Publ Hlth CIBERESP, Madrid, Spain
Celik, M:
Hacettepe Univ, Fac Med, Dept Social Pediat, Ankara, Turkey
Cetinkaya, D:
Hacettepe Univ, Fac Med, Dept Pediat Hematol, Ankara, Turkey
:
Fdn Promot Sanit & Biomed Res Valencia Reg FISABI, Area Genom & Hlth, Valencia, Spain
CIBER Epidemiol & Publ Hlth CIBERESP, Madrid, Spain
Univ Valencia, Inst Integrat Syst Biol I2SysBio, Valencia, Spain
CNR, CSIC, Valencia, Spain
Dinleyici, E:
Eskisehir Osmangazi Univ, Fac Med, Dept Pediat, Eskisehir, Turkey
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