Spectral Transmission of the Human Corneal Layers
Por:
Peris-Martínez C, García-Domene MC, Penadés M, Luque MJ, Fernández-López E and Artigas JM
Publicada:
1 oct 2021
Ahead of Print:
29 sep 2021
Categoría:
Medicine (miscellaneous)
Resumen:
We have assessed the spectral transmittance of the different layers of the human cornea in the ultraviolet (UV), visible, and near-infrared (IR) spectral ranges. Seventy-four corneal sample donors were included in the study. Firstly, the corneal transmittance was measured using a spectrophotometer. Then, all samples were fixed for histopathological analysis, which allowed us to measure the thickness of each corneal layer. Finally, the absorption coefficients of the corneal layers were computed by a linear model reproducing total transmittance. The results show that corneal transmission was almost in unity at the visible and IR ranges but not at the UV range, in which the layer with higher transmission is Descemet's membrane, whereas the stroma showed the lowest transmittance. Regarding the absorption coefficient, the most absorptive tissue was Bowman's layer, followed by the endothelium. Variations on transmittance due to changes in the stroma, Bowman's layer, or Descemet layer were simulated, and important transmission increases were found due to stroma and Bowman changes. To conclude, we have developed a method to measure the transmittance and thickness for each corneal layer. All corneal layers absorb UV light to a greater or lesser extent. The absorption coefficient is higher for Bowman's layer, while the stroma is the layer with the lowest transmittance due to its thickness. Variations in stroma thickness or changes in the corneal tissue of Bowman's layer or the endothelium layer due to some pathologies or surgeries could affect, to a greater or lesser degree, the total transmission of the cornea. Thus, obtaining accurate absorption coefficients for different layers would help us to predict and compensate these changes.
Filiaciones:
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
Surgery Department, Ophthalmology, School of Medicine, University of Valencia, Av. Blasco Ibáñez, 15, E-46010 Valencia, Spain
Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain
Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C/Sinesio Delgado, 4, E-28029 Madrid, Spain
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C/Sinesio Delgado, 4, E-28029 Madrid, Spain
Pathology Group, PASAPTA, Veterinary School, Cardenal Herrera-CEU University, C/Tirant lo Blanc, 7, E-46115, Alfara del Patriarca, E-46115 Valencia, Spain
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
:
Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmología Médica (FOM), Av. Pío Baroja, 12, E-46015 Valencia, Spain
Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain
Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C/Sinesio Delgado, 4, E-28029 Madrid, Spain
gold, Green Published
|