A new MALDI-TOF approach for the quick sequence type identification of Legionella pneumophila.
Por:
Blanco, S, Sanz, C, Gutierrez, M, Simarro, M, Lopez, I, Escribano, I, Eiros, J, Zarzosa, P, Orduna, A, Lopez, J and March, G
Publicada:
1 sep 2021
Ahead of Print:
22 jul 2021
Resumen:
BACKGROUND: Recently, MALDI-TOF has emerged as a quick tool for bacterial typing. The aim was to evaluate if MALDI-TOF based typing of Legionella pneumophila can achieve the same discriminatory power as that of the Sequence Based Typing (SBT) method. METHODS: The Sequence Type (ST) was obtained from the 90 strains included in the training set and an in-house MALDI-TOF library based on the Main Spectra Profile (MSP) was generated for the identification of such ST. Then, our library was validated by three procedures: a) creating a dendrogram, b) searching for specific peaks present exclusively in each MSP entry, and c) analysing a validation set composed of 14 strains with known ST. Fully characterized L. pneumophila ATCC 33152, which belongs to ST 36, was used as a control strain. RESULTS: In the training set, 17 strains belonged to ST 1, 1 to ST 20, 63 to ST 22, 1 to ST 146, 6 to ST 578, and 2 to ST 1086. Specific peaks present in each MSPs spectrum, which are considered type-specific biomarkers, ranged from 2 to 11; more concretely, MSP for ST 1 identification shows 2 specific peaks; MSP for ST 20 identification: 9 specific peaks; MSP for ST 22 and ST 36 identification: 11 specific peaks; MSP for ST 146 identification: 5 specific peaks; and MSP for ST 578 and ST 1086 identification: 3 specific peaks. Using the validation set (nine strains belonging to ST 22 and five to ST 1), MALDI-TOF assigned accurately the ST in 30 min per tested strain with a full match. CONCLUSIONS: The ST of L. pneumophila can be identified and reported in few minutes directly from colonies grown on BCYE agar using MALDI-TOF.
Filiaciones:
Blanco, S:
Univ Valladolid, Fac Ciencias, Dept Quim Fis & Quim Inorgan, IU CINQUIMA, Campus Miguel Delibes,Calle Paseo Belen, Valladolid 47011, Spain
Sanz, C:
Univ Salamanca, Fac Farm, Campus Miguel Unamuno,Calle Lic Mendez Nieto S-N, Salamanca 37007, Spain
Gutierrez, M:
Univ Valladolid, Fac Med, Area Microbiol, Av Ramon Y Cajal S-N, Valladolid 47003, Spain
Simarro, M:
Univ Valladolid, Fac Med, Area Microbiol, Av Ramon Y Cajal S-N, Valladolid 47003, Spain
Lopez, I:
Hosp Sierrallana, Serv Microbiol, Barrio Ganzo S-N, Torrelavega 39300, Spain
:
Hosp Virgen Los Lirios, Serv Microbiol, Calle Alcalde Pont Francisco Aura Boronat 2, Alcoy 03804, Spain
Eiros, J:
Univ Valladolid, Fac Med, Area Microbiol, Av Ramon Y Cajal S-N, Valladolid 47003, Spain
Hosp Univ Rio Hortega, Serv Microbiol & Parasitol, Calle Dulzaina 2, Valladolid 47012, Spain
Zarzosa, P:
Univ Valladolid, Fac Med, Area Microbiol, Av Ramon Y Cajal S-N, Valladolid 47003, Spain
Orduna, A:
Univ Valladolid, Fac Med, Area Microbiol, Av Ramon Y Cajal S-N, Valladolid 47003, Spain
Hosp Clin Univ Valladolid, Serv Microbiol & Inmunol, Av Ramon Y Cajal 3, Valladolid 47003, Spain
Lopez, J:
Univ Valladolid, Fac Ciencias, Dept Quim Fis & Quim Inorgan, IU CINQUIMA, Campus Miguel Delibes,Calle Paseo Belen, Valladolid 47011, Spain
March, G:
Hosp Clin Univ Valladolid, Serv Microbiol & Inmunol, Av Ramon Y Cajal 3, Valladolid 47003, Spain
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