Bacterial Composition and Metabolomics of Dental Plaque From Adolescents
Por:
Havsed K, Stensson M, Jansson H, Carda-Diéguez M, Pedersen A, Neilands J, Svensäter G and Mira A
Publicada:
30 jul 2021
Ahead of Print:
30 jul 2021
Resumen:
Supragingival dental plaque samples were collected from 40 Swedish adolescents, including 20 with caries lesions (CAR) and 20 caries-free (CF). Fresh plaque samples were subjected to an ex vivo acid tolerance (AT) test where the proportion of bacteria resistant to an acid shock was evaluated through confocal microscopy and live/dead staining, and the metabolites produced were quantified by H-1 Nuclear Magnetic Resonance (H-1 NMR). In addition, DNA was extracted and the 16S rRNA gene was sequenced by Illumina sequencing, in order to characterize bacterial composition in the same samples. There were no significant differences in AT scores between CAR and CF individuals. However, 7 out of the 10 individuals with highest AT scores belonged to the CAR group. Regarding bacterial composition, Abiotrophia, Prevotela and Veilonela were found at significantly higher levels in CAR individuals (p=0.0085, 0.026 and 0.04 respectively) and Rothia and Corynebacterium at significantly higher levels in CF individuals (p=0.026 and 0.003). The caries pathogen Streptococcus mutans was found at low frequencies and was absent in 60% of CAR individuals. Random-forest predictive models indicate that at least 4 bacterial species or 9 genera are needed to distinguish CAR from CF adolescents. The metabolomic profile obtained by NMR showed a significant clustering of organic acids with specific bacteria in CAR and/or high AT individuals, being Scardovia wiggsiae the species with strongest associations. A significant clustering of ethanol and isopropanol with health-associated bacteria such as Rothia or Corynebacterium was also found. Accordingly, several relationships involving these compounds like the Ethanol : Lactate or Succinate : Lactate ratios were significantly associated to acid tolerance and could be of predictive value for caries risk. We therefore propose that future caries risk studies would benefit from considering not only the use of multiple organisms as potential microbial biomarkers, but also their functional adaptation and metabolic output.
Filiaciones:
Havsed K:
Department of Pediatric Dentistry, Institute for Postgraduate Dental Education, Jönköping, Sweden
Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden
Section for Oral Biology and Pathology, Faculty of Odontology, Malmö University, Malmö, Sweden
Stensson M:
Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden
Folktandvården Skåne, The Swedish Dental Service of Skåne, Lund, Sweden
Jansson H:
Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden
Folktandvården Skåne, The Swedish Dental Service of Skåne, Lund, Sweden
Department of Periodontology, Faculty of Odontology, Malmö University, Malmö, Sweden
:
Department of Health & Genomics, Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation, Valencia, Spain
Pedersen A:
Swedish NMR Centre, The University of Gothenburg, Gothenburg, Sweden
Neilands J:
Section for Oral Biology and Pathology, Faculty of Odontology, Malmö University, Malmö, Sweden
Biofilms - Research Center for Biointerfaces, Malmö University, Malmö, Sweden
Svensäter G:
Section for Oral Biology and Pathology, Faculty of Odontology, Malmö University, Malmö, Sweden
Biofilms - Research Center for Biointerfaces, Malmö University, Malmö, Sweden
:
Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden
Department of Health & Genomics, Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation, Valencia, Spain
Green Published, gold, Gold, Green
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