Fluorescence in situ hybridization of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes


Por: Sanchez-Castro, J, Marco-Betes, V, Gomez-Arbones, X, Garcia-Cerecedo, T, Lopez, R, Talavera, E, Fernandez-Ruiz, S, Adema, V, Marugan, I, Luno, E, Sanzo, C, Vallespi, T, Arenillas, L, Marco-Buades, J, Batlle, A, Buno, I, Martin-Ramos, M, Blazquez-Rios, B, Collado-Nieto, R, Vargas, M, Gonzalez-Martinez, T, Sanz, G and Sole, F

Publicada: 2 nov 2015
Resumen:
Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected - 17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 0.9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p)

Filiaciones:
Sanchez-Castro, J:
 Hosp Arnau Vilanova, Lleida, Spain

Marco-Betes, V:
 Hosp Arnau Vilanova, Lleida, Spain

Gomez-Arbones, X:
 Hosp Arnau Vilanova, Lleida, Spain

 Univ Lleida, Lleida, Spain

Garcia-Cerecedo, T:
 Hosp Arnau Vilanova, Lleida, Spain

Lopez, R:
 Hosp Arnau Vilanova, Lleida, Spain

Talavera, E:
 Hosp Arnau Vilanova, Lleida, Spain

Fernandez-Ruiz, S:
 Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain

Adema, V:
 Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain

Marugan, I:
 Univ Valencia, Hosp Clin, Valencia, Spain

Luno, E:
 Hosp Univ Cent Asturias, Oviedo, Spain

Sanzo, C:
 Hosp Univ Cent Asturias, Oviedo, Spain

Vallespi, T:
 Hosp Valle De Hebron, Barcelona, Spain

Arenillas, L:
 Hosp del Mar, Barcelona, Spain

:
 Hosp Gen Castellon, Castellon de La Plana, Spain

Batlle, A:
 Hosp Univ Marques Valdecilla, Santander, Spain

Buno, I:
 Hosp Gen Univ Gregorio Maranon, Madrid, Spain

Martin-Ramos, M:
 Hosp Univ 12 Octubre, Madrid, Spain

Blazquez-Rios, B:
 Hosp Basurto, Bilbao, Spain

Collado-Nieto, R:
 Hosp Gen Univ Valencia, Valencia, Spain

Vargas, M:
 Hosp Univ Virgen Macarena, Seville, Spain

Gonzalez-Martinez, T:
 Hosp Clin Univ, Fdn Publ Galega Med Xenom, Santiago, Spain

Sanz, G:
 Hosp Univ & Politecn La Fe, Valencia, Spain

Sole, F:
 Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain
ISSN: 10428194





LEUKEMIA & LYMPHOMA
Editorial
TAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND, Reino Unido
Tipo de documento: Article
Volumen: 56 Número: 11
Páginas: 3183-3188
WOS Id: 000365520200027
ID de PubMed: 25754580
imagen Green Accepted

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