Fluorescence in situ hybridization of TP53 for the detection of chromosome 17 abnormalities in myelodysplastic syndromes
Por:
Sanchez-Castro, J, Marco-Betes, V, Gomez-Arbones, X, Garcia-Cerecedo, T, Lopez, R, Talavera, E, Fernandez-Ruiz, S, Adema, V, Marugan, I, Luno, E, Sanzo, C, Vallespi, T, Arenillas, L, Marco-Buades, J, Batlle, A, Buno, I, Martin-Ramos, M, Blazquez-Rios, B, Collado-Nieto, R, Vargas, M, Gonzalez-Martinez, T, Sanz, G and Sole, F
Publicada:
2 nov 2015
Resumen:
Conventional G-banding cytogenetics (CC) detects chromosome 17 (chr17) abnormalities in 2% of patients with de novo myelodysplastic syndromes (MDS). We used CC and fluorescence in situ hybridization (FISH) (LSI p53/17p13.1) to assess deletion of 17p in 531 patients with de novo MDS from the Spanish Group of Hematological Cytogenetics. FISH detected - 17 or 17p abnormalities in 13 cases (2.6%) in whom no 17p abnormalities were revealed by CC: 0.9% of patients with a normal karyotype, 0% in non-informative cytogenetics, 50% of patients with a chr17 abnormality without loss of 17p and 4.7% of cases with an abnormal karyotype not involving chr17. Our results suggest that applying FISH of 17p13 to identify the number of copies of the TP53 gene could be beneficial in patients with a complex karyotype. We recommend using FISH of 17p13 in young patients with a normal karyotype or non-informative cytogenetics, and always in isolated del(17p)
Filiaciones:
Sanchez-Castro, J:
Hosp Arnau Vilanova, Lleida, Spain
Marco-Betes, V:
Hosp Arnau Vilanova, Lleida, Spain
Gomez-Arbones, X:
Hosp Arnau Vilanova, Lleida, Spain
Univ Lleida, Lleida, Spain
Garcia-Cerecedo, T:
Hosp Arnau Vilanova, Lleida, Spain
Lopez, R:
Hosp Arnau Vilanova, Lleida, Spain
Talavera, E:
Hosp Arnau Vilanova, Lleida, Spain
Fernandez-Ruiz, S:
Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain
Adema, V:
Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain
Marugan, I:
Univ Valencia, Hosp Clin, Valencia, Spain
Luno, E:
Hosp Univ Cent Asturias, Oviedo, Spain
Sanzo, C:
Hosp Univ Cent Asturias, Oviedo, Spain
Vallespi, T:
Hosp Valle De Hebron, Barcelona, Spain
Arenillas, L:
Hosp del Mar, Barcelona, Spain
:
Hosp Gen Castellon, Castellon de La Plana, Spain
Batlle, A:
Hosp Univ Marques Valdecilla, Santander, Spain
Buno, I:
Hosp Gen Univ Gregorio Maranon, Madrid, Spain
Martin-Ramos, M:
Hosp Univ 12 Octubre, Madrid, Spain
Blazquez-Rios, B:
Hosp Basurto, Bilbao, Spain
Collado-Nieto, R:
Hosp Gen Univ Valencia, Valencia, Spain
Vargas, M:
Hosp Univ Virgen Macarena, Seville, Spain
Gonzalez-Martinez, T:
Hosp Clin Univ, Fdn Publ Galega Med Xenom, Santiago, Spain
Sanz, G:
Hosp Univ & Politecn La Fe, Valencia, Spain
Sole, F:
Inst Recerca Leucemia Josep Carreras IJC, Barcelona 08916, Spain
Green Accepted
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